Inhibitory effect of hypericin on intracellular proliferation of Toxoplasma gondii in macrophages
CUI Jie1,2,3, WANG Qi4, YANG Xiao-di2, SUN Xi-meng5, WANG Rui2, JIANG Hui2, WANG Shou-xiang2, ZHANG Min2, WEI Ming2, XIA Hui2, CHEN Xing-zhi2, SHEN Ji-long1
1. Department of Parasitology, School of Basic Medicine, Anhui Medical University, Hefei 230032, China; 2. Department of Microbiology and Parasitology, Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu 233030, China; 3. Microscopic Morphology Integrated Laboratory, Bengbu Medical College, Bengbu 233030, China; 4. Anhui Provincial Institute of Schistosomiasis Control, Hefei 230061, China; 5. Department of Parasitology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China
Abstract：The suppressive effect of hypericin on the intracellular proliferation of Toxoplasma gondii (T.gondii) tachyzoites in mouse peritoneal macrophages was explored in this study. Macrophages were isolated and infected with T. gondii tachyzoites (type I, RH strain) expressing green fluorescent protein (GFP). There were four groups: the control group (macrophages infected with RH-GFP without treatment, group A) and the other three groups (macrophages infected with RH-GFP treated with different concentrations of hypericin: group B, with 100 μg/mL; group C, with 200 μg/mL; and group D with 400 μg/mL). Tachyzoites were collected after 1 hour, 2 hours, and 3 hours of treatment. The infected macrophages and the free parasites were observed under different concentrations of hypericin in various time intervals by fluorescence microscopy and flow cytometry. The ultrastucture of tachyzoites was also observed by transmission electron microscopy. Compared with the control group, the number and fluorescence intensity of tachyzoites were gradually decreased in a concentration and time dependent manner under the experimental condition. The ratio of free parasites to those within macrophages increased and reached the peak at the dosage of 400 μg/mL on 3 hours after treatment. With the extension of treatment duration, it was found that tachyzoites showed swelling and deformed; apparent gap appeared between cell membrane and matrix; more and bigger vacuoles were formed; the cell membrane became dissolved and disrupt, the inner structure overflowed. It’s suggested that the inhibition of hypericin on intracellular proliferation of T. gondii tachyzoites in macrophages is presented in a concentration and time dependent manner in vitro, meanwhile, a remarkable killing effect on free tachyzoites is also found.
崔洁, 王旗, 杨小迪, 孙希萌, 汪瑞, 姜辉, 王守祥, 张敏, 魏明, 夏惠, 陈兴智, 沈继龙. 金丝桃素对小鼠巨噬细胞内刚地弓形虫速殖子的增殖抑制作用[J]. 中国人兽共患病学报, 2018, 34(7): 583-587.
CUI Jie, WANG Qi, YANG Xiao-di, SUN Xi-meng, WANG Rui, JIANG Hui, WANG Shou-xiang, ZHANG Min, WEI Ming, XIA Hui, CHEN Xing-zhi, SHEN Ji-long. Inhibitory effect of hypericin on intracellular proliferation of Toxoplasma gondii in macrophages. Chinese Journal of Zoonoses, 2018, 34(7): 583-587.
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2018, Vol. 34 
