Identification and MLVA genotyping of Bacillus anthracis strains isolated in Guizhou Province from 2012 to 2015
MA Qing1, YU Huan2, LIU Ying1, WANG Ming1, YAO Guang-hai1, TANG Guang-peng1, WANG Ding-ming1, LI Shi-jun1
1.Guizhou Provincial Center for Disease Control and Prevention, Guiyang 550004,China; 2. Zunyi Maternal and Child Care Service Center, Zunyi 563000, China
Abstract：In order to provide scientific basis for the control and prevention of anthrax by the epidemiological characteristic of Bacillus anthracis. The 409 samples including 388 from outside environment, 19 from patients and 2 from animals in different regions of Guizhou Province from 2012 to 2015 were used for B. anthracis isolatation and identification by using the traditional Gram staining, penicillin inhibition experiments and bacteriophage lysis test. The B. anthracis genome DNA was amplified using PCR based on 15 variable number of tandem repeats (VNTR), respectively. The amplification length of each VNTR locus was obtained by capillary electrophoresis. The copy numbers of tandem repeat sequence were subsequently analyzed with the software of NTsys 2.10e. The results showed that 34 B. anthracis strains were isolated from 409 specimens with positive rate of 8.31%. The positive rates of soil and skin lesion exudate samples were 9.68% (33/341) and 5.88%(1/17), respectively. Among the four years, the year 2015 was with highest positive rate of B. anthracis, with positive rate of 48.72% (19/39). MLVA-15 genotyping showed that the 34 isolates were typed into 3 MLVA-15 types. Clustering analysis showed that the 34 isolates divided into A and B clusters. Clade A was further divided into A1 and A2 clade. Most isolates come from the same region or year was closely clustered. Results of this study suggested that the soil sample was of the highest positive rate with B. anthracis among all the samples from Guizhou Province during 2012-2015 and B. anthracis isolates in Guizhou Province were of genetic diversity and were clustered geographically.
马青, 喻欢, 刘英, 王铭, 姚光海, 唐光鹏, 王定明, 李世军. 2012-2015年贵州省炭疽芽胞杆菌分离鉴定及MLVA分型分析[J]. 中国人兽共患病学报, 2018, 34(7): 624-628.
MA Qing, YU Huan, LIU Ying, WANG Ming, YAO Guang-hai, TANG Guang-peng, WANG Ding-ming, LI Shi-jun. Identification and MLVA genotyping of Bacillus anthracis strains isolated in Guizhou Province from 2012 to 2015. Chinese Journal of Zoonoses, 2018, 34(7): 624-628.
2018, Vol. 34 
