Preliminary studies on effect of pVAX1/IL-18 induced pVAX1/SjRPS4·CB vaccine immunoreaction of Schistosomiasis japonica
CHENG Hong-bing1,2, ZHOU Yun-fei1, ZHANG Shu-ju1, FENG Qi-mei1, LIU Yi-ping1,3, CUI Guo-yan1,2, WEI Hong1,4, LI Fen1, LIU Ming-she3, WANG Shi-ping1
1.Department of Parasitology, Xiangya Medical School,Central South University, Changsha 410078,China; 2.Department of Microbiology, Changzhi Medical College,Changzhi 046000,China; 3.Department of Parasitology, Changzhi Medical College,Changzhi 046000,China; 4.Department of Infection Control, the Affiliated Heping Hospital of Changzhi Medical College,Changzhi 046000,China
Abstract：In this paper, the influence of pVAX1/IL-18 as an adjuvant on the pVAX1/SjRPS4·CB vaccine against Schistosoma japonicum infection was explored. Seventy BABL/c mice were randomly divided into five groups as normal saline(NS) control group, pVAX1 group, pVAX1/IL-18 group, pVAX1/SjRPS4·CB vaccine group, pVAX1/SjRPS4·CB vaccine and pVAX1/IL-18 group. 100μg normal saline or plasmids were respectively injected intramuscularly for three times with two weeks interval. Three weeks after the final injection, each mouse was challenged with Schistosoma japonicum cercariae (20±1) infection on the abdomen. ELISA was used to detect the induced specific antibody. Adult worm burden reduction was calculated according to the adult worms in mice. Intestine eggs reduced rate, liver eggs reduced rate and per female worm eggs reduced rate were calculated according to the number of Intestine eggs, liver eggs, and per female worm eggs. Eight weeks after the infection, the IgG antibody levels of the mice with pVAX1/SjRPS4·CB vaccine and pVAX1/IL-18 group was significantly higher than other groups. Combined pVAX1/SjRPS4·CB vaccine and pVAX1/IL-18 group had shown significant decreases in adults worm burden, liver eggs, intestine eggs and uterus eggs, with those being reduced about 52%, 63%, 67% and 52%. The reduction rates in the above combined group were all significantly higher than other three experimental groups(P<0.05). pVAX1/IL-18 using as an adjuvant can increase the protection effect of SjRPS4·CB vaccine against Schistosoma japonicum infection.
[1] 雷正龙,周晓农.消除血吸虫病——我国血吸虫病防治工作的新目标与新任务[J]. 中国血吸虫病防治杂志, 2015,27(1):1-4. [2] Chen J, Pan J, Wang J, et al.Soluble egg antigens of Schistosoma japonicum induce senescence in activated hepatic stellate cells by activation of the STAT3/p53/p21 pathway[J]. Sci Rep, 2016, (6):30957e. [3] Tian Z, Wang XY, Zhou YF, et al.Schistosoma japonicum scFv-IL18 fusion DNA ameliorates hepatic fibrosis in schistosomiasis-infected mice via improving local concentration of IL-18 in liver[J]. Exp Parasitol. 2013, 134(4):447-454. [4] Tian Z, Wang XY, Zeng SH, et al.Characterization and vaccination of two novel Schistosoma japonicum genes screened from a cercaria cDNA library[J]. Parasitol Res, 2012, 110: 403-409. [5] 张丽,李文桂.日本血吸虫融合基因疫苗的研究进展[J].中国病原生物学杂志,2013,8(11) :1036-1041. [6] 彭微,汪世平,郑长黎,等.日本血吸虫pVAX1/SjRPS4·CB多价疫苗免疫对小鼠肝脏虫卵肉芽肿形成的影响[J]. 中国人兽共患病学报,2012, 28(12): 1216-1219. [7] 彭微,汪世平,郑长黎,等.日本血吸虫pVAX1/SjRPS4·CB多价疫苗免疫对小鼠肝脏虫卵肉芽肿形成中TNF-α表达的影响[J].中国人兽共患病学报,2013, 29(6):537-542. DOI:10.3969/j.issn.1002-2691.2013.06.003 [8] Min-Jong K, Robea JH, Amy G, et al.IL-18 is induced and IL-18 receptor play a critical role in the pathogenesis of cigarette smoke induced pulmonary emphysema and inflammation[J]. J lmmunol, 2007, 178(3):1948-1959. [9] Siva Reddy K, Muralidhar Rao D, Badrinaryana N, et al.Enhancement of DNA vaccine (P12A3C-pcDNA) efficacy against foot-and-mouth disease by coadministration of interleukin-18-expressing (IL18 pcDNA) plasmid in guinea-pigs[J]. Immunol Med Microbiol, 2010, 60(3):261-269. [10] Dupré L, Kremer L, Wolowczuk I, et al.Immunostimulatory effect of IL-18-encoding plasmid in DNA vaccination against murine Schistosoma mansoni infection[J]. Vaccine, 2001, 19(11-12):1373-1380. [11] Akhiani AA, Schon K, Lycke N, et al.Vaccine-induced immunity against Helicobacter pylori infection is impaired in IL-18-decient mice[J]. J Immunol, 2004, 173(5):3348-3356. [12] Bani-Hani A H, Leslie J A, Asanuma H, et al. IL-18 neutralization ameliorates obstruction-induced epithelial-mesenchymal transition and renal fibrosis[J]. Kidney international, 2009, 76(5): 500-511. [13] Eberl M, Beck E, Coulson P S, et al.IL-18 potentiates the adjuvant properties of IL-12 in the induction of a strong Th1 type immune response against a recombinant antigen[J]. Vaccine, 2000, 18(19): 2002-2008. [14] Yin RL, Li C, Yang ZT, et al.Construction and immunogenicity of a DNA vaccine containing clumping factor A of Staphylococcus aureus and bovine IL18[J]. Vet Immunol Immunopathol, 2009, 132(2/4):270-274. [15] Dinarello CA, Fantuzzi G. Interleukin-18 and host defense against infection[J]. J Infect Dis, 2003, 187 Suppl 2: S370-384. [16] Zhang LH, Pan JP, Yao HP, et al.Intrasplenic transplantation of IL-18 gene-modified hepatocytes: an effective approach to reverse hepatic fibrosis in schistosomiasis through induction of dominant Th1 response[J]. Gene Ther, 2001, 8(17): 1333-1342. [17] Palacios-Macapagal D, Connor J, Mustelin T, et al.Cutting Edge: Eosinophils Undergo Caspase-1-Mediated Pyroptosis in Response to Necrotic Liver Cells[J]. J Immunol, 2017 ,199(3):847-853. [18] Dupré L, Kremer L, Wolowczuk I, et al.Immunostimulatory effect of IL-18-encoding plasmid in DNA vaccination against murine Schistosoma mansoni infection[J]. Vaccine, 2001, 19(11-12):1373-1380. [19] Men JT, Liu Q, Shang LM, et al.IL-18 augments protective immunity of Sj23 plasmid DNA vaccine against Schistosoma japonicum in mice[J]. Chin J Vet Sci, 2009, 29(5):610-614.
