Abstract：We aimed to find out the distribution features of virulence genes sitC and iroN of Salmonella enterica isolates from diarrhea patients and healthy people in Fujian, and analyse the distribution differences of these virulence genes in the four common serotypes of Salmonella enteric in Fujian, so as to provide the basis for the further effective prevention and control of salmonellosis. A total of 166 strains of Salmonella enterica isolated from diarrhea patients (121 strains) and healthy people (45 strains) in Fujian Province were selected, including four serotypes of S. typhimus, S. enteritis, S. Del humiliation and S. Stanley. The conventional PCR method was used to amplify the virulence gene sitC and iroN. SPSS 17 was used for data statistics. Results showed that the total detection rates of virulence genes sitC and iroN in 166 isolated strains were 51.81% (86 strains) and 39.76% (66 strains), respectively. There was no significant difference in the detection rate of sitC and iroN in diarrhea patients and healthy people (χsitC2=0.347, P=0.556; χiroN2=3.322, P=0.068). While there was significant difference in the detection rate of sitC and iroN in the four serotypes of S. typhimus, S. enteritis, S. Del humiliation and S. Stanley (χsitC2=11.634, P=0.009; χiroN2=15.020, P=0.002). SitC+iroN+ and sitC-iroN- were the most common patterns of gene delivery. A certain proportion of sitC and iroN were detected in Salmonella isolates from Fujian Province. The distribution of these two virulence genes was different from serotypes, but there was no difference in the distribution of Salmonella isolates between healthy people and diarrhea patients. So the Salmonella isolates from healthy people in Fujian Province have strong survival ability and certain pathogenicity potential, as well as Salmonella isolates from diarrhea patients.
黄梦颖, 陈建辉, 邱玉锋, 杨劲松, 陈爱平. 福建省沙门菌毒力基因sitC及iroN分布特征分析[J]. 中国人兽共患病学报, 2018, 34(9): 783-787.
HUANG Meng-ying, CHEN Jian-hui, QIU Yu-feng, YANG Jin-song, CHEN Ai-ping. Distribution features of Salmonella virulence genes sitC and iroN in Fujian, China. Chinese Journal of Zoonoses, 2018, 34(9): 783-787.
[1] 陈明明,王芯,葛美霞. 从业人员肠道沙门菌检测结果分析及PCR技术的应用[J]. 微量元素与健康研究. 2017,34(6):43-46. [2] 方艳红,孙裴,魏建忠,等. 沙门菌毒力基因研究进展[J]. 动物医学进展. 2010,31(S):190-193. [3] 杨劲松,陈爱平,陈建辉,等. 福建省沙门菌病invA与spvB基因分布状况的研究[J]. 海峡预防医学杂志. 2009,15(1):53-55. [4] Skyberg JA, Logue CM, Nolan LK.Virulence genotyping of Salmonella spp. with multiplex PCR[J]. Avian Dis, 2006, 50(1): 77-81. DOI: 10.1637/7417.1. [5] Janakiraman A, Slauch JM.The putative iron transport system SitABCD encoded on SPI1 is required for full virulence of Salmonella typhimurium[J]. Mol Microbiol, 2000, 35(5): 1146-1155. [6] Zhou D, Hardt WD, Galán JE.Salmonella typhimurium encodes a putative iron transport system within the centisome 63 pathogenicity island[J]. Infect Immun, 1999, 67(4): 1974-1981. [7] Bäumler AJ, Norris TL, Lasco T, et al.IroN, a novel outer membrane siderophore receptorcharacteristic of Salmonella enteric[J]. J Bacteriol, 1998, 180(6): 1446-1453. [8] Borges KA, Furian TQ, de Souza SN, et al. Phenotypic and molecular characterization of Salmonella enteritidis SE86 isolated from poultry and salmonellosis outbreaks[J]. Foodborne Pathog Dis, 2017, 14(12): 742-754. DOI: 10.1089/fpd.2017.2327. [9] 熊海平,汤敏,张宏萍,等. 南通市沙门菌毒力基因分布特征的初步研究[J]. 齐齐哈尔医学院学报. 2009,30(19):2355-2357. [10] Tamang MD, Gurung M, Nam HM, et al.Antimicrobial susceptibility and virulence characteristics of Salmonella enterica Typhimurium isolates from healthy and diseased pigs in Korea[J]. J Food Prot. 2014, 77(9):1481-1486. DOI: 10.4315/0362-028X.JFP-14-084 [11] Krawiec M, Kuczkowski M, Kruszewicz AG, et al.Prevalence and genetic characteristics of Salmonella in free-living birds in Poland[J]. BMC Vet Res, 2015, 11:15. DOI: 10.1186/s12917-015-0332-x [12] Kim JE, Lee YJ.Molecular characterization of antimicrobial resistant non-typhoidal Salmonella from poultry industries in Korea[J]. Ir Vet J, 2017, 70: 20. DOI: 10.1186/s13620-017-0095-8 [13] Nde CW, Logue CM.Characterization of antimicrobial susceptibility and virulence genes of Salmonella serovars collected at a commercial turkey processing plant[J]. J Appl Microbiol, 2008, 104(1): 215-223. DOI: 11.1111/j.1365-2672.2007.03535.x [14] 谢一俊,郑国櫆,陈建辉,等. 福建省1975-2003年沙门菌监测结果分析[J]. 海峡预防医学杂志. 2004,10(6):7-10. [15] 陈建辉,欧剑鸣,杨劲松,等. 2006~2011年福建省沙门菌监测菌株血清型分布及耐药性分析[J]. 预防医学论坛. 2014,20(2):81-87. [16] 邱玉锋,陈建辉,黄梦颖,等. 2010-2016年福建省部分腹泻患者沙门菌血清型、耐药监测[J]. 预防医学论坛. 2017,23(7):489-491. [17] 陈建辉,黄梦颖,杨劲松,等. 1993年-2015年福州市服务行业人员沙门菌携带情况监测分析[J]. 中国卫生检验杂志. 2016,26(14):2081-2084.
2018, Vol. 34 
