Effects of the polarization of the macrophage differentiated from THP-1 which infected with Toxoplasma gondii
LIU Xue-lei1, WANG Yun-jie1, YANG Ning-ai1, AN Tong-tong1, KANG Yu-ting1, JIA Wei2, 3, SU Ya-jing3, ZHAO Zhi-jun2, 3
1. College of Clinical Medical, Ningxia Medical University, Yinchuan 750004, China; 2. Laboratory Medicine Center, General Hospital of Ningxia Medical University, Yinchuan 750004, China; 3. Ningxia Key Laboratory of Clinical and Pathogenic Microbiology, Yinchuan 750004, China
Abstract：To explore the effect of the polarization of the macrophage differentiated from THP-1 which infected with Toxoplasma gondii, monocytes (THP-1) suspended in the culture medium were induced by phorbol ester (PMA) for 48 h to differentiate into adherent macrophages. In vitro experiment at different T. gondii infection time course and the protozoan proliferation rate was studied by using Diff staining. Western blot and Q-PCR were used to detect the expression level of polarization-related proteins and mRNA. The protein expression level of iNOS in M1 macrophage was significantly lower than that in the control group, and the expression of M2 protein Arg-1 was the highest expressed with the highest level at 36 h. Results of Q-PCR showed that the mRNA expression of IL-1, IL-12, iNOS and TNF-α in different treatment groups gradually decreased, but IL-10 and Arg-1 gradually increased and peaked at 36 h (t=9.587, P<0.05). T. gondii can proliferate in the macrophages differentiated from THP-1 cells. After T. gondii infection, it will polarize towards M2 macrophages.
刘学雷, 王云杰, 杨宁爱, 安童童, 康宇婷, 贾伟, 苏雅静, 赵志军. 弓形虫感染对THP-1巨噬细胞极化影响的研究[J]. 中国人兽共患病学报, 2018, 34(10): 902-907.
LIU Xue-lei, WANG Yun-jie, YANG Ning-ai, AN Tong-tong, KANG Yu-ting, JIA Wei, SU Ya-jing, ZHAO Zhi-jun. Effects of the polarization of the macrophage differentiated from THP-1 which infected with Toxoplasma gondii. Chinese Journal of Zoonoses, 2018, 34(10): 902-907.
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2018, Vol. 34 
