Identification of 14 cases of imported Plasmodium ovale wallikeri subspecies in Shandong Province
LI Jin, WEI Qing-kuan, XU Chao, XIAO Ting, HUANG Bing-cheng, KONG Xiang-li, WANG Long-jiang, WANG Yong-bin, XU Yan, YIN Kun, SUN Hui
Shandong Academy of Medical Sciences;Shandong Institute of Parasitic Diseases, Shandong Provincial Reference Laboratory for Malaria Diagnosis, Jining 272033, China
Abstract：To identify and analyze 14 cases of imported Plasmodium ovale wallikeri in Shandong Province, a total of 14 cases of imported malaria cases were collected. The detection was conducted by rapid malaria diagnostic test strip test (RDT), blood smear microscopy, NP-1993 conventional nested PCR identification and P. ovale wallikeri subspecies specific PCR test. After amplification, the PCR product was sequenced and Blast analysis was performed. The results of RDT in 14 cases showed that 12 cases were Pan positive and 2 case was negative. Microscopic examination indicated 13 cases were Plasmodium ovale and 1 case was Plasmodium vivax. The results of NP-1993 conventional nested PCR showed that 14 samples were negative for four kinds of Plasmodium spp. The specific PCR results showed that all the 14 samples could be amplified 780 bp with the specificity of P. ovale wallikeri subspecies. After sequencing, Blast analysis was performed and the result was P. ovale wallikeri subspecies. The 14 cases are P. ovale wallikeri subspecies infection, which are detected positive for microscopy, and negative results detected by NP-1993 conventional nested PCR.
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2018, Vol. 34 
