Effect of multilocular cyst fluid on IDO expression in mouse bone marrow derived dendritic cells
FU Yong1,2, MENG Ru3, JIANG Tao4, ZHANG Zhuang-zhi5, WANG Hong-bin6
1.Academy of Animal and Veterinary Medicine, Qinghai University, Xining 810016, China;
2.State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, China;
3.Animal Husbandry and Veterinary Station of Xining, Xining 810016,China;
4.The First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China;
5.Veterinary Research Institute, Xinjiang Academy of Animal Sciences,Urumqi 830000,China;
6. The Affiliated Hospital of Qinghai University, Xining 810001, China
Abstract:The objective of this study was to explore the ability of multilocular cyst fluid (MCF) to induce IDO expression in mouse bone marrow derived dendritic cells (BMDCs). BMDCs were obtained by induction culture in vitro, and coculture with MCF (MCF group), rmIFN-gamma (positive control group) and RPMI-1640 culture medium (negative control group). BMDCs of each group were collected at different time points. Real-time PCR method was used to detect IDO mRNA transcriptional level on BMDCs. Western blotting method was used to detect IDO protein level on BMDCs. ELISA method was used to detect the variation of IDO concentration in BMDCs supernatant. The results showed that MCF group of relative expression of IDO mRNA on BMDCs and IDO concentration in BMDCs supernatant reached peak value at 24 h, which were greatly higher than the negative control group(F=303.305, 5 752.473, P<0.01). While MCF group of relative expression level of IDO protein on BMDCs reached the highest value at 48 hours, which were significantly higher than that in negative control group (F=70.971, P<0.01). These results indicate that MCF has the ability to up regulate the expression of IDO on BMDCs, which provides a theoretical basis for further revealing the molecular mechanism of immune tolerance in alveolar echinococcosis.
[1] Kawamura N, Kamiyama T, Sato N, et al.Long-term results of hepatectomy for patients with alveolar echinococcosis: a single-center experience[J]. J Am Coll Surg, 2011, 212(5): 804-812. DOI: 10.1016/j.jamcollsurg.2011.02.007
[2] 徐士梅, 赵殷奇, 朱明星, 等. 细粒棘球蚴特异性诊断抗原Eg-07279的制备及免疫原性研究[J]. 中国人兽共患病学报, 2018, 34(2):118-123.DOI: 10.3969/j.issn.1002-2694.2018.00.012
[3] Mcmanus DP, Li Z, Yang S, et al.Case studies emphasising the difficulties in the diagnosis and management of alveolar echinococcosis in rural China[J]. Parasites & Vectors, 2011, 4(1): 196-200. DOI: 10.1186/1756-3305-4-196
[4] Siracusano A, Delunardo F, Teggi A, et al.Cystic echinococcosis: aspects of immune response, immunopathogenesis and immune evasion from the human host[J]. Endocrine, 2012, 12(12): 16-23. DOI: 10.2174/187153012799279117
[5] Manicassamy S, Pulendran B.Dendritic cell control of tolerogenic responses[J]. Immunol Rev, 2011, 241(1): 206-227. DOI: 10.1111/j.1600-065X.2011.01015.x
[6] Sher A, Pearce E, Kaye P.Shaping the immune response to parasites: role of dendritic cells[J]. Curr Opin Immunol, 2003, 15(4): 421-429. DOI: 10.1016/S0952-7915(03)00072-4
[7] Wang Y, Zhou HJ, Shen YJ, et al.Impairment of dendritic cell function and induction of ${CD}_{4}^{+}$ CD25+Foxp3+T cells by excretory-secretory products: a potential mechanism of immune evasion adopted by Echinococcus granulosus[J]. BMC Immunology, 2015, 16(1): 1-10. DOI: 10.1186/s12865-015-0110-3
[8] 单骄宇, 李海涛, 李春燕, 等. 不同棘球蚴抗原诱导树突状细胞表达吲哚胺2, 3-双加氧酶的实验研究[J].中国寄生虫学与寄生虫病杂志, 2013, 31(3): 188-192.
[9] Mandal S, Mandal DM.Human cystic echinococcosis: epidemiologic, zoonotic, clinical, diagnostic and therapeutic aspects[J]. Asian Pac J Trop Med, 2012, 5(4): 253-260. DOI: 10.1016/S1995-7645(12)60035-2
[10] McManus DP, Zhang W, Li J, et al. Echinococcosis[J]. Lancet, 2003, 362(9392): 1295-1304. DOI:10.1016/S0140-6736(03)14573-4
[11] 沈定文,罗金萍,王若愚,等. 树突状细胞多价核酸疫苗抗血吸虫感染作用及机制研究[J]. 中国人兽共患病学报, 2016, 32(4): 406-411.DOI:10.3969/j.issn.1002-2694.2016.04.017
[12] Quan S, Kim HJ, Dukala D, et al.Impaired dendritic cell function in a spontaneous autoimmune polyneuropathy[J]. J Immunol, 2015, 194(9): 4175-4184. DOI: 10.4049/jimmunol.1401766
[13] Heitger A.Regulation of expression and function of IDO in human dendritic cells[J]. Curr Med Chem, 2011, 18(15): 2222-2233. DOI: 0929-8673/11 $58.00+.00
[14] 单骄宇, 李海涛, 吐尔洪江·吐逊, 等. 细粒棘球蚴抗原诱导树突状细胞表达吲哚胺2, 3-双加氧酶的动态观察[J]. 中国病原生物学杂志, 2012, 7(9): 688-692.
[15] Riganò R, Buttari B, De Falco E, et al.Echinococcus granulosus-specific T-cell lines derived from patients at various clinical stages of cystic echinococcosis[J]. Parasite Immunol, 2004, 26(1): 45-52. DOI: 10.1111/j.0141-9838.2004.00682.x