Serotype dstribution and antimicrobial resistance and antimicrobial resistance genes of Escherichia coli isolated from raw livestock and poultry products in Guangxi
ZHONG Shu-hong1, LI Jun1, FENG Shi-wen1, ZHOU Qing-an2, CHEN Ze-xiang1, XU Li-shi3, LIU Feng1, MA Chun-xia1, HU Shuai1, PAN Yan1
1.Guangxi Veterinary Research Institute, Guangxi Key Laboratory of Veterinary Biotechnology, Nanning 530001, China; 2. Guangxi Center for Animal Disease Control and Prevention, Nanning 530001, China; 3. College of Animal Science and Technology, Guangxi University, Nanning 530005, China
Abstract:To investigate the serotype distribution and the antimicrobial resistance with the prevalence of resistance genes of pathogenic Escherichia coli isolated from raw livestock and poultry products in Guangxi, the “O” antigen serotype of 249 pathogenic E.coil stains were detected using the agglutination method. The antimicrobial resistance were determined by antibiotic susceptibility test according to the K-B method.The blaTEM, blaCTX-M, oqxA, oqxB, qnrA and aac(6')-Ⅰb-cr genes were screened by PCR.Results showed that among the 249 strains of E. coli,136 strains were classified,96 strains were untyped, and 17 strains were self-agglutinated, accounting for 54.6% (136/249), 38.6% (96/249), and 6.8% (17/249), respectively. The 136 classified strains were belong to 25 serotypes with the main predominant serotypes were O8 19.85%(27/136), O86 8.82%(12/136), O6 6.62%(9/136), O28 6.62%(9/136) and O124 6.62%(9/136). The 136 established strains of E. coli showed different resistance levels to the 24 antibiotics with the resistance rate were 16.18 to 100%, and all of them were multiple drug resistance as they resisted 6 antibiotics in minimum and 22 antibiotics in maximum and majority of them resisted 12 to 18 antibiotics which accounted for 72.06% (98/136). The PCR results showed that the detection rate of the six resistant genes in the 136 strains were qnrA 91.18%(124/136), oqxA 80.88%(110/136), oqxB 50.00%(68/136), aac(6')-Ib-cr 35.29%(48/136), blaTEM 100.00%(136/136), and blaCTX-M 23.53%(32/136). These indicate that serotypes of E.coil in animal products in Guangxi distribute in a variety of ways. The antimicrobial resistance of the established strains were very serious, and there is a relationship between the antimicrobial resistance with the prevalence of drug resistance genes.
钟舒红, 李军, 冯世文, 周庆安, 陈泽祥, 许力士, 柳锋, 马春霞, 胡帅, 潘艳. 广西生鲜畜禽产品源致病性大肠杆菌血清型、耐药性及耐药基因调查[J]. 中国人兽共患病学报, 2018, 34(11): 1010-1015.
ZHONG Shu-hong, LI Jun, FENG Shi-wen, ZHOU Qing-an, CHEN Ze-xiang, XU Li-shi, LIU Feng, MA Chun-xia, HU Shuai, PAN Yan. Serotype dstribution and antimicrobial resistance and antimicrobial resistance genes of Escherichia coli isolated from raw livestock and poultry products in Guangxi. Chinese Journal of Zoonoses, 2018, 34(11): 1010-1015.