Abstract:We analyzed the stability of the recombinant Lactococcus lactis(L.lactis) vaccine of Taenia solium TSOL18 gene after the artificial generation. These two plasmids of pMG36e-TSOL18 and pMG36e-SP-TSOL18 were electroporated into L.lactis MG1363. Positive clones were identified by PCR. In the absence of erythromycin resistance and erythromycin resistance under the condition of continuous artificial passage for 20 times, the stability of pMG36e-TSOL18 and pMG36e-SP-TSOL18 in L.lactis was analyzed through the determination of plasmid genetic stability rate and SacI/HindIII enzyme digestion. After pMG36e-TSOL18 and pMG36e-SP-TSOL18 were continuously passaged for 20 generations in L.lactis, the stability rate of these two plasmids was 100% in the absence of erythromycin resistance, and the stability rate was 99% under the erythromycin resistance. These plasmids of all generations were identified by restriction endonuclease SacI/HindIII. The agarose gel electrophoresis showed that the size was correct, and it was consistent with the original plasmid when it was continuously transmitted to the 20th generation. It was indicated that pMG36e-TSOL18 and pMG36e-SP-TSOL18 plasmid had good genetic stability when they were continuously passaged for 20 generations in L.lactis, which laid the foundation for further research on TSOL18 gene recombinant L.lactis vaccine of Taenia solium.
周必英, 孙俊超. 猪带绦虫TSOL18基因重组乳球菌疫苗的稳定性分析[J]. 中国人兽共患病学报, 2018, 34(11): 1016-1020.
ZHOU Bi-ying, SUN Jun-chao. Stability of the recombinant Lactococcus lactis vaccine of Taenia solium TSOL18 gene. Chinese Journal of Zoonoses, 2018, 34(11): 1016-1020.
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