1. National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; 2. Jiangxi Center for Disease Control and Prevention, Nanchang 330029, China; 3. Southwest university, Chongqing 400715, China
Abstract:In order to explore the molecular and serological characterization of pathogenic Leptospira in Jiangxi Province, a collection of 27 strains isolated from Jiangxi Province in China since 2013 were analyzed using Serogroup identification, 16S rRNA gene sequencing and multilocus sequence typing (MLST) in this study. Seven loci were chosen for genotyping in MLST analysis and the results were analyzed by BioNumerics (Version 5.10) softwares. Microscopic agglutination test (MAT) was conducted and a total of four serogroups were identified among 27 Leptospira strains. Serogroup Icterohaemorrhagiae was the most frequent serogroup, accounting for 59.26%, followed by Javanica 25.92%, Australis 7.41% and Bataviae 7.41%, respectively. 16S rRNA sequencing was conducted and two pathogenic species of L. interrogans and L. borgpetersenii were identified among the 27 isolates. L. interrogans was the most prevalence species, accounting for 77.78%, while the remaining 6 isolates were identified as pathogenic L. borgpetersenii. Five different sequence types (STs) were obtained By MLST. ST1 accounted for 59.26% and was the major genotype in Jiangxi Province in China. Phylogenetic analysis using BioNumerics softwares revealed that five major clusters (Cluster1 to Cluster5) corresponding to five ST types was identified among 27 isolates. Obvious epidemiological relationship was found between the distributions of STs and isolated location, but not across years. In this work, the results revealed that MLST analysis could be used to explore the genetic diversity and population structures of Leptospira. In this work, the results revealed that Serogroup Icterohaemorrhagiae was the most frequent serogroup, L. interrogans was the most prevalence species, and ST1 was the major genotype in Jiangxi Province in China. The detailed serological and molecular characteristics circulating in this region may provide new insights into the epidemiology of leptospirosis and vaccine design in China.
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