Genotyping and drug resistance of 127 strains of Mycobacterium tuberculosis
LIU Yi-ping1, PANG Hui2, WAN Kang-lin3, GONG Yan-e4, GUO Xu-xia4, WANG Yan-xia4, LIU Hai-can3
1. Department of Medical Parasitology, Changzhi Medical College, Changzhi 046000, China; 2. Department of Immunology, Changzhi Medical College, Changzhi 046000, China; 3. State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; 4. Department of Inspection, Heping Hospital Affiliated to Changzhi Medical College, Changzhi 046000, China
Abstract:To understand the genetic polymorphism and the correlation of genotype and drug resistance of Mycobacterium tuberculosis (M. tuberculosis) circulating in Changzhi and the surrounding area in Shanxi Province, multi-loci variable number tandem repeats (VNTRs) analysis (MLVA) were utilized for the genotyping of the clinical isolates and drug susceptibility test (DST) of the 8 antituberculosis agents was performed on the Lowenstein-Jensen (L-J) medium by the proportion method. The BioNumerics (Version 5.0) software was used for clustering analysis. The result showed that Mtub21, MIRU26 and ETRE revealed high polymorphism (Hunter-Gaston Index, HGI>0.6). MIRU40, ETRB, MIRU16, ETRD, MIRU23 and ETRC showed low polymorphism (HGI<0.3). The 127 M. tuberculosis strains were divided into 11 groups of which the groupⅠ accounting for the largest percentage of 80.31% and 102 genotypes including 90 idiotopes. The drug resistance of the first-line antituberculosis agents and the second-line antituberculosis drugs were 47.24% and 23.62%. Among the first-line antituberculosis agents, streptomycin (SM) (40.94%) had the highest drug resistance, and ethambutol (EMB) (6.30%) was the best antibacterial agents against experimental strains. Of the second-line antituberculosis drugs, protionamide (PTH) (3.15%) was the good choice with the lowest drug resistance, and levofloxacin (LFX) (14.17%) had the highest drug resistance. The single drug resistance of streptomycin (11.81%) was the highest followed by isoniazid (2.36%), and we found that no strains were drug resistant for single rifampicin or single ethambutol. The 21.26% (27/127) were multi-drug resistant, and the 3.15% were widely multi-drug resistant. It’s no statistical significance that the difference of the drug resistance rate between the groupⅠ strains and other strains. In conclusion, it’s preliminarily confirmed that the M. tuberculosis has obvious genetic polymorphism and the group Ⅰ strains were popular in Changzhi and the surrounding area. The sensitivity to the second-line antituberculosis drugs was higher than that to the first-line antituberculosis drugs. It had no obvious correlation between the major epidemic strains groupⅠand drug resistance.
刘益萍, 庞慧, 万康林, 弓艳娥, 郭旭霞, 王艳霞, 刘海灿. 127株结核分枝杆菌基因分型及耐药相关研究[J]. 中国人兽共患病学报, 2018, 34(12): 1119-1125.
LIU Yi-ping, PANG Hui, WAN Kang-lin, GONG Yan-e, GUO Xu-xia, WANG Yan-xia, LIU Hai-can. Genotyping and drug resistance of 127 strains of Mycobacterium tuberculosis. Chinese Journal of Zoonoses, 2018, 34(12): 1119-1125.