MLST typing and genetic evolution of Campylobacter jejuni isolated from poultry in some areas of Sichuan,China
YAO Xue-ping1,2, ZHANG Bo1, WANG Han1, WU Xu-long1, ZHOU Li-jun1, CAO Sui-zhong1,2, WANG Yin1,2, YANG Ze-xiao1,2, DEXI Cuo-mu3
1.College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China; 2.Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China; 3. Animal Husbandry Station of Tibet Chang Zuogong County, Zuogong 854400, China
Abstract：To determine the molecular characteristics of 48 strains of Campylobacter jejuni isolated from some parts of Sichuan, the molecular typing and genetic evolution of Campylobacter jejuni were analyzed. According to MLST database the selection of Campylobacter jejuni MLST genotyping of 7 housekeeping genes aspA, glnA, glyA, gltA, tkt, pgm and uncA, the sequencing as target genes were amplified, and the sequences were compared with phylogenetic analysis. The result showed that there were 24 different sequences in 48 isolates, belonging to 9 different clones and 11 unique types, among which 22 of the isolates contained a new sequence, accounting for 45.83% (22/48). Among the 9 different clones, CC-21, CC-353, and CC-464 clones contained more strains relatively, while the clones CC-45 and CC-48 contained one isolated. The 11 unique types coexist in 21 isolates, of which 8 strains were the ST-8675 sequence types. Phylogenetic tree showed that the isolates belonging to different clones of the same clonal line have close genetic relationships, like the ST-298 and ST-760 isolates belonging to the CC-21 clonal line were on the same small branch and closely related. However, the genetic relationship between the isolates of different clonal lines was far away. The quail source isolates (V13-20) were in a large branch and has the most distant relationship with other poultry isolates. Overall, isolates from different regions and host sources showed genetic diversity. Gene polymorphism exists in the isolates in this study, and MLST typing provides a reference for understanding the genetic characteristics and distribution characteristics of Campylobacter jejuni in the area.
姚学萍, 张博, 王寒, 邬旭龙, 周丽军, 曹随忠, 王印, 杨泽晓, 德西措姆. 四川部分地区禽源空肠弯曲菌MLST分型及遗传进化分析[J]. 中国人兽共患病学报, 2019, 35(1): 5-6.
YAO Xue-ping, ZHANG Bo, WANG Han, WU Xu-long, ZHOU Li-jun, CAO Sui-zhong, WANG Yin, YANG Ze-xiao, DEXI Cuo-mu. MLST typing and genetic evolution of Campylobacter jejuni isolated from poultry in some areas of Sichuan,China. Chinese Journal of Zoonoses, 2019, 35(1): 5-6.
2019, Vol. 35 
