PCR specie identification and phylogenetic position analysis for microorganism in tapeworm from chicken
LIU Jing-ying1, LIANG Yue-qin1, GUO Yong-qiang1, CUI Li-juan1, XUE Huan1, ZHANG Ding2
1. Function Laboratory, Shanxi Medical University, Taiyuan 030001, China; 2. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China
Abstract：To identify the bacteria and fungi species that were parasitic on Raillietina echinobothrida found in chicken, the present study designed the bacteria 16s rDNA and fungi 18s rDNA universal primer respectively. After extracting the genomic DNA of Raillietina echinobothrida, target genes were amplified by RT-PCR and then sequenced. Additionally, the phylogenetic trees of target gene sequences were constructed by neighbor-joining method and genetic distance were analyzed by MEGA5.1 software. Results showed that the bacteria parasitic on Raillietina echinobothrida belonged to Achromobacter sp. and the identity were 99%. The fungi were identified as Malassezia sp. in the known species and genomic homology was the closest to the Malassezia restricta, reaching to 99%. In conclusion, the present study firstly identified the bacteria and fungi species in Raillietina echinobothrida by PCR method, and the results are expected to provide an effective reference for the prevention and treatment of secondary infection in chicken caused by Raillietina echinobothrida.
刘晶英, 梁月琴, 郭永强, 崔丽娟, 薛欢, 张鼎. 鸡绦虫寄生菌种的PCR鉴定及其进化关系分析[J]. 中国人兽共患病学报, 2019, 35(1): 11-14.
LIU Jing-ying, LIANG Yue-qin, GUO Yong-qiang, CUI Li-juan, XUE Huan, ZHANG Ding. PCR specie identification and phylogenetic position analysis for microorganism in tapeworm from chicken. Chinese Journal of Zoonoses, 2019, 35(1): 11-14.
2019, Vol. 35 
