Antitumor activity of Vibrio parahaemolyticus thermostable direct hemolysin on human lung cancer cell
MA Yan1, YANG Jing-ya1,2,3, LI Shi-yi1, YU Wen-juan4
1. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China; 2. Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation,Shanghai Ocean University, Shanghai 201306, China; 3. Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, Shanghai 201306, China; 4. College of Aqua-life Science and Technology, Shanghai Ocean University, Shanghai 201306, China
Abstract：Anti-tumor of marine bacterial toxin is a new idea for the treatment of cancer in recent years. The aim of this study was to investigate the inhibitory effects of thermostable direct hemolysin (TDH) on human lung cancer cell line SPC-A-1. TDH was separated and purified from marine bacteria-Vibrio parahaemolyticus ATCC33846 metabolites (TDH) by DEAE-cellulose, DEAE-Sephadex A-50 and Sephadex G-75 chromatography. In this paper, the comparison of the concentration of semi inhibitor in different cells treated by TDH, the effect of TDH on human lung cancer cell line SPC-A-1 was studied by MTT assay, cell scratch experiment, apoptosis experiment and caspase-3 activity experiment for the first time. It showed that the IC50 of human colon epithelial cells NCM460, human liver fibroblast cells LO2 and human lung cancer cell SPC-A-1 treated by TDH with 24 h were 151μg /mL, 118μg /mL and 6.7μg /mL, IC50 of the normal cells is higher than that of lung cancer cell about 20 times. The results of growth curve and wound test showed that TDH could inhibit the growth, proliferation and migration of SPC-A-1 cells in a dose-dependent manner below 5 μg/mL. Further flow cytometry revealed that 5 μg/mL TDH induced early apoptosis in 33% of SPC-A-1 cells. These results suggest that TDH may inhibit the growth and proliferation of SPC-A-1 cells by inducing apoptosis.
马燕, 杨靖亚, 李诗怡, 喻文娟. 副溶血弧菌耐热直接溶血毒素抑制人肺腺癌细胞活性的研究[J]. 中国人兽共患病学报, 2019, 35(1): 54-58.
MA Yan, YANG Jing-ya, LI Shi-yi, YU Wen-juan. Antitumor activity of Vibrio parahaemolyticus thermostable direct hemolysin on human lung cancer cell. Chinese Journal of Zoonoses, 2019, 35(1): 54-58.
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