Abstract:The aim of this study is to identify quickly and accurately genotype of Mycobacterium avium subsp. paraenberculosis and provide reference to the prevention and control of paratuberculosis, DNA was extracted from two suspected samples of red deer paratuberculosis. Primers were designed based on IS900 of M. avium subsp. paraenberculosis according to reference. The amplified fragments were sequenced. Analysis of the nucleotide sequences and construction of phylogenetic tree were based on IS900 gene of Mycobacterium avium subsp. paraenberculosis with computer programs. The sequencing results showed that two suspected samples had 99% similarity to reference strain from GenBank. Two suspected samples were tested to infect M. avium subsp. paraenberculosis. According to DMC primers, the pathogen was genotype Ⅱ Mycobacterium avium subsp. paraenberculosis. It was confirmed that two red deer infected genotype Ⅱ paratuberculosis according to symptoms, pathological change, acid-fast stain and PCR.
王娜, 朱广艺, 艾克拜尔·热合曼, 崔鑫, 胡建军, 张莹钰, 张婉琪. 基于PCR检测和基因测序分析的鹿源副结核分枝杆菌鉴定及亚型分型[J]. 中国人兽共患病学报, 2019, 35(1): 85-90.
WANG Na, ZHU Guang-yi, AIKEBAIER·Reheman, CUI Xin, HU Jian-jun, ZHANG Ying-yu, ZHANG Wan-qi. Genotype identification of Mycobacterium avium subsp. paraenberculosis from Red Deer based on PCR and sequence analysis. Chinese Journal of Zoonoses, 2019, 35(1): 85-90.
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