Comparative proteomics analysis of the virulent promastigotes and avirulent promastigotes of a Leishmania strain from Jiashi, Xinjiang, China
WEI Fu-rong, GAO Chun-hua, WANG Jun-yun, YANG Yue-tao, SHI Feng
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Laboratory of Parasite and Vector Biology, Ministry of Public Health, National Center for International Research on Tropical Diseases, WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, China
Abstract:We analyzed the protein abundance differences between the virulent promastigotes and avirulent promastigotes in the same Leishmania strain by comparative proteomics method. Tryptic digests of total proteins were analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS), followed by Label-free quantitative differential expression analysis. The MS data were analyzed with MaxQuant software (ver 1.3.0.5) against database. The mass spectrometric analysis of each sample was repeated for three times. Only the proteins that were present in all three replicates (LFQ intensity) for at least one condition were included in the data set. This study resulted in the identification of 3 994 proteins across 2 samples. Of these, 298 differentially expressed proteins (ratio>1.2 or ratio <0.83, P<0.05) were identified in at least two of the three technical replicates with two or more LFQ intensity number considered. Among these differentially expressed proteins, 15 proteins were uniquely in the virulent promastigotes, 23 proteins in the avirulent promastigotes. Of 260 differentially expressed proteins were the same in the two groups, among which 162 proteins were down-regulated and 78 proteins were up-regulated based on protein annotation. Some of the identified differentially expressed proteins were demonstrated and they involved in biological functions such as glucose and lipid metabolism, nucleotide acid metabolism, stress response, cytoskeleton, cell cycle and proliferation. It is evident that different protein expression profile of the virulent promastigotes and avirulent promastigotes in the same Leishmania strain and this finding may lay the foundation for screening and identification of the key leishmanial molecules for infecting mammal host cells.
危芙蓉, 高春花, 汪俊云, 杨玥涛, 石锋. 我国利什曼原虫伽师株有毒力和无毒力前鞭毛体定量蛋白质组学比较分析[J]. 中国人兽共患病学报, 2019, 35(2): 97-102.
WEI Fu-rong, GAO Chun-hua, WANG Jun-yun, YANG Yue-tao, SHI Feng. Comparative proteomics analysis of the virulent promastigotes and avirulent promastigotes of a Leishmania strain from Jiashi, Xinjiang, China. Chinese Journal of Zoonoses, 2019, 35(2): 97-102.
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