Abstract:To analyze the pathogen spectrum of hand, foot, and mouth disease (HFMD) and to investigate the molecular epidemiology, genetic variation and evolution of coxsackievirus A16 (CVA16) strains were analyze for the data on epidemiological characteristics and pathogens detected results of HFMD cases in Jiangsu province from 2009 to 2017. The complete VP1 sequences of 120 CVA16 strains were amplified and sequenced for the homological and phylogenetic analysis. Results showed that a total of 1 013 771 suspected HFMD patients were reported in Jiangsu province from 2009 to 2017, and the positive enterovirus samples were 37 389. EV71 (30.13%) and CVA16 (30.13%) were the predominant pathogens among these cases. The major severe cases were EV71. The CVA16 infection showed two peaks during April-July and September-November. The major CVA16 infectious population was focused on the ages ranged from one to four years old with higher incidence in males than in females. The 120 CVA16 strains were divided into subgenotype of B1a and B1b, and the B1b subgenotype was the predominant genotype of Jiangsu CVA16 strains. The nucleotide and amino acid identity among the CVA16 strains were 87.5%~100% and 97%~100%, respectively. Results indicate that epidemiology characteristics of CVA16 in Jiangsu is significant, 120 strains belong to B1a and B1b subgenotype, and they are random nucleotide sequences of CVA16 isolates are varied greatly, but the homology only distributed in mild cases and severe cases based on phylogenetic tree analysis. The amino acid is comparatively high. Thus, it is indicated the existence of some synonymous mutations.
嵇红, 鲍倡俊, 祁贤, 霍翔, 胡建利, 樊欢. 2009-2017年江苏省手足口病病原谱及柯萨奇A16型的分子流行病学研究[J]. 中国人兽共患病学报, 2019, 35(2): 113-119.
JI Hong, BAO Chang-jun, QI Xian, HUO Xiang, HU Jian-li, FAN Huan. Pathogen spectrum of hand, foot and mouth disease and molecular epidemiology of coxsackievirus A16 strains in Jiangsu province, China, 2009-2017. Chinese Journal of Zoonoses, 2019, 35(2): 113-119.
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