Abstract:To study the difference in the growth curves and expressionlevels of mazEF3,6,9 systems between Beijing and non-Beijing genotype Mycobacterium tuberculosis (MTB) strains,growth curves of Beijing and non-Beijing genotype strains as well as their mazEF3, mazEF6, mazEF9 genes deletion and overexpressing were surveyed and drawn in standard, hypoxia and nutrient starvation culture conditions. The mRNA expression levels of mazEF3, 6, 9, the mazF3, 6, 9 toxin genes, and the mazE3, 6 and 9 antitoxin genes in all strains were detected using Quantitative real time-PCR (qRT-PCR). Expression level of the MazF9 was determined by Western Blot. Results showed that OD values (numbers of the strains ) of Beijing genotype strains were higher than that of non-Beijing genotype strains on the 4th, 6th and 10th days under standard and hypoxia culture conditions, and on the 2nd, 4th and 8th day under nutritional hunger culture condition, differences were significant (P<0.05). The numbers of Beijing and non-Beijing genotype strains with mazEF3, mazEF6, mazEF9 genes deletion and overexpressing were fewer than that of their parents under the above three culture conditions. At the mRNA and protein levels, compared with non-Beijing genotype strains, the mazEF3 has a higher expression of 9.55 times, a mazF3 higher expression of 4.86 times, a mazF6 higher expression of 2.64 times, a mazF9 higher expression of 5.27 times, a mazE9 lower expression of 0.16 times and mazF9 protein is higher expression 1.62 times in Beijing genotype strains, and the differences were statistically significant. There are significant differences in the growth curves and mazEF system expression levels between Beijing and non-Beijing genotype strains. MazEF system is related to the prevalence of Beijing genotype strains.
谢婉莹, 赵继利, 杨赛丽, 刘微, 袁俐. 北京和非北京基因型结核分枝杆菌生长曲线及mazEF系统的研究[J]. 中国人兽共患病学报, 2019, 35(6): 502-508.
XIE Wan-ying, ZHAO Ji-li, YANG Sai-li, LIU Wei, YUAN Li. Growth curves and expression of Mycobacterium tuberculosis Beijing and non-Beijing genotype and the mazEF system. Chinese Journal of Zoonoses, 2019, 35(6): 502-508.
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