Clinical evaluation of MTBDRplus linear probe assay for rapid detection of multidrug-resistant Mycobacterium tuberculosis
PAN Jie-ru1, YE Hai-mei1, WU Zhu-da1, ZHANG Bao-hua2, ZHENG Hou-jian3
1.Fuzhou Center for Disease Control and Prevention, Fuzhou 350004, China; 2. The Second Department of Clinical Laboratory of 900 Hospital of the Joint Logistics Team, Fuzhou 350003, China; 3. Lianjiang Center for Disease Control and Prevention, Lianjiang 350500, China
Abstract:To evaluate the efficiency of MTBDRplus linear probe assay (MTBDRplus) for detecting multidrug resistant (MDR) Mycobacterium tuberculosis (TB) in Fuzhou. A total of 246 clinical TB isolates were included in the study. Traditional Lwenstein-Jensen culture plus drug sensitivity test was used as the gold standard to evaluate the results of the new MTBDRplus. The variety of gene mutations were also assessed. The sensitivity of MTBDRplus for detection of resistance to Rifampicin (RIF) and Isoniazide (INH) were 91.7% (11/12) and 83.3%(15/18). The specificity of MTBDRplus for detection of RIF and INH were 99.6% (233/234) and 95.2% (217/228). Among them, 58.3% were S531L mutation in 12 TB cases with rpoB gene mutation. Of the 26 cases of TB with gene mutations of katG and inhA, 57.7%(15/26) was S315T mutation of katG gene. Only 9.1% (1/11) of isolates with C15T mutation were the INH resistant strains. MTBDRplus technology is a sensitive and specific method for rapid diagnosis of MDR-resistant tuberculosis. The technology has a good prospect in Fuzhou area. The resistant strains of TB in Fuzhou were mainly rpoB S531L and katG S315T mutations. It is recommended to carefully determine the INH resistance of M. tuberculosis caused by inhA gene C15T mutation.
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