Multilocus sequence typing of Burkholderia pseudomallei collected in Hainan, China
LIU Lin1,2,SUN Qing-hui1,2,PEI Hua1,3,CHEN Chui-zhe1,2, XIU Hao1,2,ZHANG Nan1,2,HE Na1,2,XIA Qian-feng1,2
1.Laboratory of Tropical Biomedicine and Biotechnology, Hainan Medical College, Haikou 571101, China; 2.Faculty of Tropical Medicine and Laboratory Medicine, Hainan Medical College, Haikou 571101; 3.Department of Clinical Laboratory, The Second Affiliated Hospital of Hainan Medical University,Haikou 571101
Abstract：This work aims to study the polymorphism of the multilocus sequence typing of 18 Burkholderia pseudomallei in Hainan province and to understand the difference in genetic background of the isolates in other endemic areas. Fragments of seven housekeeping genes including ace,glt B,gmh D,lep A,lip A,nar K and ndh were amplified and the obtained sequences were compared using MLST website. 18 isolates of B.pseudomallei were used and it was found 12 STs were identified, among which a new ST1676 was identified in this study. Among these STs, ST50 recovered 22.2% (4 cases ), ST58 (2), ST232 (2), ST1094 (2), and one case of each remaining STs. Overall these result, we concluded Hainan province B.pseudomallei is highly diverse. GmhD is the most variable allele of the seven housekeeping genes, suggested that variation in this allele may be responsible for the high genetic diversity of STs observed in this study.
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2019, Vol. 35 
