Confirmation of one human Monkeypox infection in Sierra Leone in 2017 by molecular and serological detection
YE Fei1,2, SONG Jing-dong1,2, ZHAO Li1, ZHANG Yi1,2, XIA Lian-xu2, ZHU Ling-wei2, REN Jiao1, WANG Wen-ling1, WU Gui-zhen1, TIAN Hou-wen1, TAN Wen-jie1
1. Key Laboratory of Medical Virology, National Health Commission, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; 2. Sierra Leone-China Friendship Biological Safety Laboratory,Freetown 999127,Sierra Leon
Abstract:To diagnosis the suspected monkeypox (MPX) patient in southern Sierra Leone, March 2017,sera samples from suspected patients were collected 12 days (SL-D12) and 55 days (SL-D55) after infection. Real-time PCR was performed by using monkeypox virus (MPXV)-specific primers, and sera antibodies were detected by Orthopoxvirus (OPV) specific-IgM, IgG and MPXV-specific IgG enzyme-linked immnosorbent assay (ELISA). Confirmation of MPVX infection was further combined with epidemiological information. The real-time PCR results revealed that SL-D12 serum was positive for MPXV infection. Vaccinia virus IgG, IgM and MPXV virus IgG antibody in SL-D12 and SL-D55 serum were detected as positive. Furthermore, MPXV-specific IgG ELISA assay shown that the SL-D12 serum was positive to an 800-fold dilution and the SL-D55 serum to a dilution of 3 200. Thus, an increase in the MPXV-specific antibody response of over fourfold was evident during recovery. The patient was conformed as a MPXV infection by molecular and serological detection combined with the clinical characteristics and the contact with wild animal in Sierra Leone in 2017.
叶飞, 宋敬东, 赵莉, 张益, 夏连续, 祝令伟, 任娇, 王文玲, 武桂珍, 田厚文, 谭文杰. 分子与血清学检测确诊2017年西非塞拉利昂1例猴痘病毒感染病例[J]. 中国人兽共患病学报, 2019, 35(6): 535-538.
YE Fei, SONG Jing-dong, ZHAO Li, ZHANG Yi, XIA Lian-xu, ZHU Ling-wei, REN Jiao, WANG Wen-ling, WU Gui-zhen, TIAN Hou-wen, TAN Wen-jie. Confirmation of one human Monkeypox infection in Sierra Leone in 2017 by molecular and serological detection. Chinese Journal of Zoonoses, 2019, 35(6): 535-538.
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