Antimicrobial resistance features and molecular typing of Staphylococcus aureus in Jiangsu Province,China
HONG Jie1, CAO Yang2, TAN Zhong-min1, QIAN Hui-min1, DONG Chen1, GU ling1, KONG Xiao-xiao1, ZHOU Lu1
1.Intestinal Pathogenic Microorganisms Key Laboratory of Health Department, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009; 2.Nanjing Medical University, Nanjing 210029
Abstract：In order to study the characteristics of drug resistance, resistance genes and genotype of Staphylococcus aureus in different areas of Jiangsu Province, a total of 227 Staphylococcus aureus from each hospital were collected during January 2017 and December 2017. Fifteen Antimicrobial minimal inhibitory concentrations were detected by method of micro-dilution broth. Seven different resistance genes were detected by PCR. Molecular typing was performed using multilocus sequence typing (MLST). Results indicated that the 227 strains of S.aureus showed high drug resistance rate to penicillin, erythromycin, cefoxitin, oxacillin, clindamycin, levofloxacin, tetracycline and gentamycin. Though the strains were sensitive to teicoplanin, rifampin, sulfamethoxazole, daptomycin, linezolid and macrodantin, the drug resistance rates were less than 10%. The results also revealed that all isolates were sensitive to vancomycin. Of 117 strains of MRSA were found that the average drug-resistance types and the carrying number of the drug-resistance genes higher than those of MSSA (P<0.05). The strains from north of Jiangsu Province exhibited a more number of drug-resistance than midland and south, while drug-resistance genes of Midland had more quantity than the south. Sixty STs were discovered in the 227 strains of S.aureus, and twenty-nine of them were novel. ST59 and ST398 distribute the largest number in all isolates. Clone complex 59 (CC59) indicated a higher number of drug-resistance and detected rate of MRSA than any other clone complex. In conclusion, the isolates of S.aureus from different area in Jiangsu Province display differences between drug-resistance rate and drug-resistance mechanism, and the strains of CC59 had a grim situation to drug-resistance.
洪捷,曹阳,谈忠鸣,钱慧敏,董晨,顾玲,孔筱筱,周璐. 江苏地区金黄色葡萄球菌耐药及分子分型研究[J]. 中国人兽共患病学报, 2019, 35(7): 604-609.
HONG Jie, CAO Yang, TAN Zhong-min, QIAN Hui-min, DONG Chen, GU ling, KONG Xiao-xiao, ZHOU Lu. Antimicrobial resistance features and molecular typing of Staphylococcus aureus in Jiangsu Province,China. Chinese Journal of Zoonoses, 2019, 35(7): 604-609.
2019, Vol. 35 
