Abstract：In order to evaluate the infection of Echinococcus granulosus in dogs in the echinococcosis epidemic areas, we extracted DNA from canine faeces using the Qiagen DNeasy Powersoil kit and screened the whole mitochondrial ND6 gene as a target gene from the complete mitochondrial genomes of E.granulosus for detection and genotyping of E.granulosus. The results showed that the specificity of the PCR assay was very high, only the E.granulosus could be successfully amplified. And the analytical sensitivity was 4 pg of DNA. Moreover, the assay could amplify the target gene in faeces of the 13th day as early as possible when the dogs were infected with about 50 000 protoscoleces. In addition, in 40 faeces which were from echinococcosis epidemic areas, 6 samples provided specific DNA fragments using the Copro-PCR assay. The sequence data showed that 6 samples were identified as G1 genotype, which were the same as the sequencing results and the nucleotide sequence analysis of the part of the mitochondrial cytochrome C oxidase subunit 1 (COX1) gene-a good candidate in the genotyping of E.granulosus. The Copro-PCR assay developed in this study has high specificity and sensitivity and could be used to detect E.granulosus latent infection in dogs. In addition, the PCR-positive production also could be used for genotyping and population genetic structure analysis of E.granulosus via the nucleotide sequence analysis.
詹佳飞,宋宏宇,王凝,郭承,李春燕,谢跃,古小彬,杨光友. 基于线粒体ND6基因检测犬感染细粒棘球绦虫的粪便PCR方法[J]. 中国人兽共患病学报, 2019, 35(7): 626-632.
ZHAN Jia-fei, SONG Hong-yu, WANG Ning, GUO Cheng, LI Chun-yan, XIE Yue, GU Xiao-bin, YANG Guang-you. Detection of Echinococcus granulosus infection in dogs on the mitochondrial ND6 gene by Copro-PCR assay. Chinese Journal of Zoonoses, 2019, 35(7): 626-632.
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2019, Vol. 35 
