Receptor binding specificity of P[9] rotavirus and the correlation of its serum antibodies with Histo-Blood group antigens
HOU Yu-zhen1, LONG Yan2, GUO Lun-ai2, CHEN Jun-rui2, ZHANG Xu-fu2, DAI Ying-chun1
1.Department of Epidemiology, School of Public Health, Southern Medical University, Guangzhou 510515, China; 2. School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong 510515, China
Abstract:We express the GST-VP8* proteins of P[9] rotavirus and explore its binding pattern with HBGA receptor. We detect P[9] rotavirus antibody in Shanwei area of Guangdong, and analyze the association of positive antibody rate with HBGA receptor. The recombinant plasmid pGEX-4T-1- VP8* was conducted and expressed. The binding pattern of P[9] RV GST-VP8* proteins and HBGA receptor were identified by enzyme-linked immunosorbent assay(ELISA). The HBGA phenotypes of the individuals in Shanwei of Guangdong were determined in Saliva samples. P[9] RV IgG antibodies in sera was measured by ELISA using P[9]VP8* proteins as capture antigens to analysis the correlation between P[9] RV IgG and HBGA. In this study, [9] RV VP8* proteins were successfully expressed. P[9] RV VP8* proteins bind to type A secretor, but not to B、O secretor or all nonsecretor. The correlation between positive rate of P[9] specific IgG in serum and host Lewis and secretor phenotypes has been found among 206 studied serum samples, which would help further understanding of rotavirus epidemiology and provides a scientific basis to prevent and control rotavirus.
侯玉珍, 龙艳, 郭伦爱, 陈俊锐, 张绪富, 戴迎春. P[9]轮状病毒受体结合特征及其人群抗体水平与HBGA相关性研究[J]. 中国人兽共患病学报, 2019, 35(8): 688-693.
HOU Yu-zhen, LONG Yan, GUO Lun-ai, CHEN Jun-rui, ZHANG Xu-fu, DAI Ying-chun. Receptor binding specificity of P[9] rotavirus and the correlation of its serum antibodies with Histo-Blood group antigens. Chinese Journal of Zoonoses, 2019, 35(8): 688-693.
[1] Troeger C, Blacker BF, Khalil IA, et al. Estimates of the global, regional, and national morbidity, mortality, and aetiologies of diarrhoea in 195 countries: a systematic analysis for the Global Burden of Disease Study 2016[J]. Lancet Infect Dis, 2018, 18(11): 1211-1228. DOI: 10.1016/S1473-3099(18)30362-1 [2] Stencel-Baerenwald JE, Reiss K, Reiter DM, et al. The sweet spot: defining virus-sialic acid interactions[J]. Nat Rev Microbiol, 2014, 12(11): 739-749. DOI: 10.1038/nrmicro3346 [3] Trojnar E, Sachsenr Der J, Twardziok S, et al. Identification of an avian group A rotavirus containing a novel VP4 gene with a close relationship to those of mammalian rotaviruses[J]. J Gen Virol, 2013, 94(Pt 1): 136. DOI: 10.1099/vir.0.047381-0 [4] Rojas M, Goncalves JL, Dias HG, et al. Whole-genome characterization of a Peruvian alpaca rotavirus isolate expressing a novel VP4 genotype[J]. Vet Microbiol, 2016, 196: 27-35. DOI: 10.1016/j.vetmic.2016.10.005 [5] Huang P, Xia M, Tan M, et al. Spike protein VP8* of human Rotavirus recognizes histo-blood group antigens in a type-specific manner[J]. J Virol, 2012, 86(9): 4833-4843. DOI: 10.1128/JVI.05507-11 [6] Marionneau S, Cailleau-Thomas A, Rocher J, et al. ABH and Lewis histo-blood group antigens, a model for the meaning of oligosaccharide diversity in the face of a changing world[J]. Biochimie, 2001, 83(7): 565-573. DOI: 10.1016/S0300-9084(01)01321-9 [7] Van Trang N, Vu HT, Le NT, et al. Association between norovirus and rotavirus infection and histo-blood group antigen types in Vietnamese children[J]. J Clin Microbiol, 2014, 52(5): 1366-1374. DOI: 10.1128/JCM.02927-13 [8] Wang YH, Pang BB, Ghosh S, et al. Molecular epidemiology and genetic evolution of the whole genome of G3P[8] human rotavirus in Wuhan, China, from 2000 through 2013[J]. PLoS One, 2014, 9(3): e88850. DOI: 10.1128/JCM.02927-13 [9] Zhou N, Lv D, Wang S, et al. Continuous detection and genetic diversity of human rotavirus A in sewage in eastern China, 2013-2014[J]. Virol J, 2016, 13(1): 153. DOI:10.1186/s12985-016-0609-0 [10] Chen YH, Chen F, Zhou T, et al. Prevalence and clinical profile of rotavirus A infection among diarrhoeal children and phylogenetic analysis with vaccine strains in Chengdu, West China, 2009-2014[J]. Trop Med Intl Health, 2018, 23(7): 704-713. DOI: 10.1111/tmi.13077 [11] Griffin DD, Nakagomi T, Hoshino Y, et al. Characterization of nontypeable rotavirus strains from the United States: identification of a new rotavirus reassortant (P2A[6],G12) and rare P3[9] strains related to bovine rotaviruses[J]. Virology, 2002, 294(2): 256-269. DOI: 10.1006/viro.2001.1333 [12] Zhang XF, Long Y, Tan M, et al. P[8] and P[4] Rotavirus infection associated with secretor phenotypes among children in South China[J]. Sci Rep-UK, 2016, 6: 34591. DOI: 10.1038/srep34591 [13] Liu Y, Huang P, Tan M, et al. Rotavirus VP8*: phylogeny, host range, and interaction with histo-blood group antigens[J]. J Virol, 2012, 86(18): 9899-9910. DOI: 10.1128/JVI.00979-12 [14] Liu N, Xu Z, Li D, et al. Update on the disease burden and circulating strains of rotavirus in China: A systematic review and meta-analysis[J]. Vaccine, 2014, 32(35): 4369-4375. DOI: 10.1016/j.vaccine.2014.06.018 [15] Tan M, Jiang X. Histo-blood group antigens: a common niche for norovirus and rotavirus[J]. Expert Rev Mol Med, 2014, 16: e5. DOI: 10.1017/erm.2014.2 [16] Wang YH, Pang BB, Zhou X, et al. Complex evolutionary patterns of two rare human G3P[9] rotavirus strains possessing a feline/canine-like H6 genotype on an AU-1-like genotype constellation[J]. Infect Genet Evol, 2013, 16: 103-112. DOI: 10.1016/j.meegid.2013.01.016 [17] Tomova ES, Sarafian VS. Expression of histo-blood group antigens in vertebrate gonads[J]. Acta Biol Hung, 2010, 61(1): 64-72. DOI: 10.1556/ABiol.61.2010.1.7