Development and application of PCR method for the detectionof Campylobacter fetus
TANG Hong1,2, XU Zhong-lan3, KONG Ke1, TANG Hai-yan1, FAN Zheng-yang1, JIAO Xin-an1,2, HUANG Jin-lin1,2
1. Key Laboratory of Zoonoses of Jiangsu Province, Yangzhou University, Yangzhou 225009, China; 2. Co-Innovation Center for Prevention and Control of Important Animal Infection Diseases and Zoonoses of Jiangsu Province, Yangzhou 225009, China; 3. Yangzhou maternal and child care service center, Yangzhou 225001, China
Abstract:To establish a rapid detection assay for Campylobacter fetus, a PCR method was developed using a pair of specific primers targeting the surface protein gene sapB2 of Campylobacter fetus. Species-specific product could be detected after amplification of the DNA template of Campylobacter fetus, while other reference strains could not be detected. The detection limit of the PCR assay was 0.23 pg/μL DNA template of Campylobacter fetus pure cell culture. And it could detect as few as 0.9 CFU/mL and 20 CFU/g of Campylobacter fetus in artificial contaminated sewage and cow feces respectively. A total of 400 fresh cow anal swab samples and 125 of vaginal swab samples were detected for Campylobacter fetus by the PCR, and the result was consistent with that of the national standard method for Campylobacter fetus. These results suggest that the PCR assay can be used to rapidly detect Campylobacter fetus and show high specificity, sensitivity and being easy to handle. It provides technical support for the rapid detection of Campylobacter fetus, and it is of great significance to the diagnosis and control of Campylobacter fetus diseases.
唐虹, 徐仲兰, 孔科, 唐海燕, 范正杨, 焦新安, 黄金林. 胎儿弯曲菌PCR检测方法的建立及其初步应用[J]. 中国人兽共患病学报, 2019, 35(8): 694-698.
TANG Hong, XU Zhong-lan, KONG Ke, TANG Hai-yan, FAN Zheng-yang, JIAO Xin-an, HUANG Jin-lin. Development and application of PCR method for the detectionof Campylobacter fetus. Chinese Journal of Zoonoses, 2019, 35(8): 694-698.