Abstract:We study the molecular differences and evolution relationships of clinical and food isolates of Listeria monocytogenes with same molecular types in Hangzhou. The genome block collinearity, non-coding RNAs and virulence genes distribution were analyzed by using high-throughput genome sequencing and bioinformatics analysis method for one clinical and six food-borne ST8 strains with same pulse-field gel electrophoresis (PFGE) patterns. Genomic phylogeny was applied to trace relationships by combining ST8 strains in NCBI Assembly database. Results showed that the genome of seven isolates in Hangzhou was consistently linear. These strains differed in virulence genes lmo0036 and lmo2396 and the distribution of ncRNAs rli48, rli62, rliG and plasmid pLmA144. The genomic SNPs phylogenetic tree showed ST8 strains a high degree of homology. The closest to the clinical strain 12-103 was the food isolate 12-004 which differed only by 16 SNPs. A large group of ST8 L. monocytogenes with high genetic similarity exists in the meat market in Hangzhou in recent years. The infection of clinical isolate is very likely from this group of strains.
俞骅, 董华丽, 汪皓秋, 楼秀芹, 刘涛, 张蔚, 王旭初, 潘劲草. 杭州地区相同分子型别的单核细胞增生李斯特菌临床和食品分离株基因组分析[J]. 中国人兽共患病学报, 2019, 35(8): 699-705.
YU Hua, DONG Hua-li, WANG Hao-qiu, LOU Xiu-qin, LIU Tao, ZHANG Wei, WANG Xu-chu, PAN Jing-cao. Genomic analysis of Listeria monocytogenes of clinical and food isolates of same molecular type in Hangzhou,China. Chinese Journal of Zoonoses, 2019, 35(8): 699-705.
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