Analysis on the relationship between β-lactam resistance phenotype and ESBLs resistance gene of Salmonella typhimurium in Fujian,China
QIU Yu-feng1, CHEN Jian-hui1, HUNG Meng-yin1, YANG Jin-song1, LIN Jie1, CHAN Ai-ping1,2
1.Fujian Center for Disease Control and Prenention, Fuzhou 350001, China; 2.Fujian Provincial Key Laboratory of Zoonosis Disease Research, Fuzhou 350001, China
Abstract：To investigate the prevalence of β-lactam resistance and relationship between resistance genotype of ESBLs and resistance phenotype in Salmonella typhimurium strains in Fujian. The minimal inhibitory concentration (MIC) of 83 strains of Salmonella typhimurium against β-lactam and ESBLs strains against other 18 drugs was determined by broth dilution and the drug resistance rate was calculated. The gene of blaTEM, blaCTX-M, blaOXA and blaSHV related to ESBLs in Salmonella typhimurium strains detected by PCR, then the blaCTX-M genotype was further confirmed by sequenciny.The strains from healthy persons and diarrheal patients were shown in different resistant rate to lactam drugs,with higher rate in diarrheal patients than others. Different degrees of resistance to third-generation and fourth-generation cephalosporins were found in strains from various diarrheal patients. 10 ESBLs strains in Salmonella typhimurium were detected from diarrheal patients, the detection rate was 16.39% (10/61), and they’re all multi-drug resistance. The detection rate of blaTEM, blaCTX-M and blaOXA gene of Salmonella typhimurium in diarrhea patients were 55.74%, 12.2% and 19.67% respectively. The detection rate of blaTEM, blaCTX-M and blaOXA gene of Salmonella typhimurium in healthy persons were 55.74%, 12.2% and 0% respectively. No blaSHV gene was detected in both healthy persons and diarrhea patients. Among blaCTX-M genes positive strains, 4 belongs to blaCTX-M-14 genotype, 3 belongs to blaCTX-M-15 and blaCTX-M-55 genotypes respectively. Correlation analysis indicated that blaTEM genes was better consistency with ampicillin resistant phenotype(P<0.001, Ka=0.546). The blaCTX-M gene was highly consistency with the cefotaxime and cefepime resistant phenotypes(P<0.001, Ka=1.000), and blaCTX-M gene also was better consistency with ceftazidime(P<0.001, Ka=0.626) and aztreonam(P<0.001, Ka=0.668) resistant phenotypes. The resistance of Salmonella typhimurium to cephalosporins and ESBLs was mainly mediated by blaCTX-M gene and the multi-drug resistance of ESBLs strains is serious in Fujian now.
邱玉锋, 陈建辉, 黄梦颖, 杨劲松, 林杰, 陈爱平. 福建省鼠伤寒沙门菌β-内酰胺耐药表型及ESBLs耐药基因分析[J]. 中国人兽共患病学报, 2019, 35(10): 944-949.
QIU Yu-feng, CHEN Jian-hui, HUNG Meng-yin, YANG Jin-song, LIN Jie, CHAN Ai-ping. Analysis on the relationship between β-lactam resistance phenotype and ESBLs resistance gene of Salmonella typhimurium in Fujian,China. Chinese Journal of Zoonoses, 2019, 35(10): 944-949.
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2019, Vol. 35 
