Gene environmental structure of CTX-M-14 type ESBL gene produced by Klebsiella pneumoniae
LIU Bao-guang1, 2, WANG Bao-ying1, 2, LI Yu-cheng1, 2, BAI Ming1, 2, MIAO Ming-san1, XU Er-ping2
1. Scientific Research and Experiment Center, Henan University of Chinese Medicine, Zhengzhou 450046, China; 2. Key Laboratory for Modern Research on Zhongjing’s Herbal Formulae of Henan Province, Zhengzhou 450046, China
Abstract：To study the genetic context surrounding blaCTX-M-14 in Klebsiella pneumoniae, and to explore the transmission mechanism of the resistant gene, the conjugation assay was performed and the blaCTX-M-14-carrying transconjugant was screened. The plasmid in the transconjugant was extracted and digested. The purified restriction fragment of plasmid inserted into PBR322 vector, and the positive recombinant plasmid was screened and sequenced. The results showed that blaCTX-M-14 could be successfully transferred to the recipient strain, and the susceptibility results of the corresponding transconjugant was consistent with that of the parental strain to most of the tested antibacterial drugs. A 2637bp fragment including blaCTX-M-14 in the positive recombinant plasmid was obtained and submitted to GenBank under the accession number with HQ650134. The sequence analysis showed that the ISEcp1 and IS903 elements were present in the upstream and downstream region of blaCTX-M-14 respectively. The multidrug resistance of the blaCTX-M-14-carrying K. pneumoniae, and the self-transmissible capacity of blaCTX-M-14 remind us that it is necessary to strengthen the monitoring of antimicrobial resistance in future studies.
刘保光, 汪保英, 栗俞程, 白明, 苗明三, 许二平. 肺炎克雷伯菌产CTX-M-14型超广谱β-内酰胺酶的基因环境研究[J]. 中国人兽共患病学报, 2019, 35(10): 950-956.
LIU Bao-guang, WANG Bao-ying, LI Yu-cheng, BAI Ming, MIAO Ming-san, XU Er-ping. Gene environmental structure of CTX-M-14 type ESBL gene produced by Klebsiella pneumoniae. Chinese Journal of Zoonoses, 2019, 35(10): 950-956.
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