College of Animal Science and Veterinary Medicine, Henan Agricultural University/ International Joint Research Laboratory for Zoonotic Diseases of Henan Province, Zhengzhou 450002, China
Abstract:To investigate the expression of TLRs of HCT-8 cells infected in the early phase of Cryptosporidium parvum IId subtype (Chinese epidemic strain), the RNA of HCT-8 cells were extracted at 4 hours and 12 hours post infection. The expression of TLRs was detected by qRT-PCR. Bioinformatics analyses were used to analyze the relationship between the differentially expressed miRNAs and TLRs. TLR1-TLR10 was expressed in HCT-8 cells. Only TLR2 and TLR4 were significantly up-regulated in the early phase (4 h and 12 h) of C. parvum IId subtype infection. TLR2 and TLR4 are not the target gene of the differentially expressed miRNAs, however, these differentially expressed miRNAs could be targeting 27 genes in TLRs signaling pathway. In conclusion, TLR2 and TLR4 of HCT-8 cells may play an important role in the infection of C. parvum IId subtype, these differentially expressed miRNAs could be targeting TLR signaling pathway related genes.
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