Prevalence and drug-resistance mechanism of ESBLs in Escherichia coli isolates of pet origins
YANG Shou-shen1, 2, WANG Jia-hui1, 2, LIN Min1, 2, ZENG Xiao-fei1, 2, QIU Min-hua1, 2, CHEN Wen-yan1, 2, HE Yu-qin1, 2, LIN Zhao-sheng3, LIN Wei-ming1, 2
1. College of Life Sciences, Longyan University, Longyan 364012, China; 2. Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan 364012, China; 3. First Hospital of Longyan, Longyan 364000, China
Abstract：In order to understand the risk of the third-generation cephalosporin widely used in pets on public health, we studied the prevalence of antimicrobial resistance, distributive characteristics of extended-spectrum-β-lactamaeses (ESBLs) and molecular propagation mechanisms of the Escherichia coli isolated from pets, for providing the guidance for risk estimation. A total of 56 strains of Escherichia coli were isolated from 92 anal swabs of sick dogs and cats collected from 4 animal hospitals in Guangzhou in 2014. The susceptibility of the isolates to 17 antimicrobials was determined by ager dilution method. Phylogenetic group analysis was analyzed by PCR. The trans-conjugants were obtained by conjugative transfer test, and the replicon types for trans-conjugants were determined. Except colistin (7.1%), cefoxitin (16.1%) and ceftazidime (10.7%), resistance rates of the 11 antimicrobial agents were more than 50%, and the cefotaxime-resistance and ceftiofur-resistance rates were 55.4% and 57.1%. The results of ESBLs showed that 13 strains carried CTX-M, with the detection rate of 23.2% (13/56), and the popular subtypes were CTX-M-14, CTX-M-65 and CTX-M-55.7 CTX-M-positive trans-conjugants were obtained with main replicon type of FIB. The TEM, OXA and SHV were not detected. The isolates were mainly belonged to phylogenetic group A and group B1. In conclusion, the strains isolated from sick pets anal swab were shown in severe resistance situation, which majorly belonged to symbiotic Escherichia coli. The main mechanisms of resistance to third generation cephalosporins were CTX-M-14, CTX-M-65 and CTX-M-55. These results suggest that there is a risk of cephalosporin-resistant E. coli transmission between pets and humans.
杨守深, 王佳慧, 林敏, 曾晓菲, 邱敏华, 陈文燕, 何玉琴, 林钊盛, 林炜明. 宠物源大肠杆菌产ESBLs流行性调查与耐药机制研究[J]. 中国人兽共患病学报, 2019, 35(12): 1110-1116.
YANG Shou-shen, WANG Jia-hui, LIN Min, ZENG Xiao-fei, QIU Min-hua, CHEN Wen-yan, HE Yu-qin, LIN Zhao-sheng, LIN Wei-ming. Prevalence and drug-resistance mechanism of ESBLs in Escherichia coli isolates of pet origins. Chinese Journal of Zoonoses, 2019, 35(12): 1110-1116.
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