Abstract：The aim of this study is to screen the specific adaptor of Cronobacter sakazakii for future development of rapid detection technique. Based on comparative genomics analysis of C.sakazakii strains, protein sub cellular localization prediction and protein trans membrane regions prediction, genes mRNA expression level assay, gene clone and protein over expression, certain outer membrane protein was selected and prepared as target protein for adaptor screening. Subsequently, purified target outer membrane protein was bound to carboxyl magnetic beads to screen ssDNA adaptor. The binding affinity of adaptor to target protein and capture specificity for C.sakazakii strain were assayed. It was demonstrated that adaptorS1 presented relatively high affinity with target protein and high capture capability for C.sakazakii strain. Predicted secondary structure of S1 and S2 revealed that loop and hairpin existed generously in these adaptors.
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2020, Vol. 36 
