Genomic polymorphism of Yersinia pestis strains isolated from the wild rodents plague focus in Jianchuan, Yunnan,China
SHI Li-yuan, DING Yi-bo, ZHANG Hai-peng, GUO Ying, DUAN Cun-juan, TAN Hong-li, DONG Shan-shan, ZHONG You-hong, WANG Peng
Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Yunnan Collaborative Innovation Center of Public Health and Disease Control and Prevention, Yunnan Institute for Endemic Diseases Control and Prevention, Dali 671000, China
Abstract：In order to elucidate the evolution of Yersinia pestis population in the wild rodents plague focus in Jianchuan and its genetic relationship with strains in other plague foci in Yunnan Province, we randomly selected 24 strains of Yersinia pestis isolated from Jianchuan in different time-periods, sites and sources for genomic polymorphism analysis. Strains were divided into clusters based on both different region (DFR) and clustered regularly interspaced short palindromic repeats (CRISPRs), and the clusters were analyzed based on multiple-locus variable-number tandem repeat analysis (MLVA) once again. The results showed that among the 24 Jianchuan isolates, 20 isolates were clustered into Jianchuan Wild Rodents Plague Cluster. 3 isolates in 2017 outbreak were clustered into Lijiang Wild Rodents Plague Cluster, with two VNTR loci difference from Lijiang strain. The remaining one was a Single. The difference of VNTR loci between the strains in the 1950s and the neighboring strains in the 1980s was less than 3. In summary. First, the genotypes of Jianchuan strains form an independent gene cluster in Yunnan, and the Jianchuan cluster appeared latter than Lijiang cluster from the view of evolution. Second, the discovery of the wild rodents focus in Jianchuan was back to the 1950s. Third, the Jianchuan outbreak in 2017 belongs to Lijiang plague. There are two types of wild rodent foci in Jianchuan County. Therefore, the dynamic surveillance of plague among wild rodents should be kept on strengthening.
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