Abstract：To explore the feasibility of DNA barcode technology for molecular biological identification and make up for the defects of morphological identification. Combined with plague monitoring work, rat-shaped animals were collected by sampling method,5-meter cage and night trapping method. After preliminary morphological identification of all specimens, molecular identification was carried out based on the mitochondrial cytochrome C oxidase I subunit gene (COI gene).And then the genetic distance was calculated and Neighbor-Joining tree was constructed. The COI gene sequences of 709 mouse-shaped animals were obtained, belonging to 38 species of 24 generas, 10 families and 3 orders. The COI gene sequences showed that the intra-species genetic distance was within 3.6%, with an average of 1.4%, the inter-species genetic distance was greater than 5.6%, the inter-species genetic distance was significantly greater than the intra-species genetic distance, and the boundaries between genera and families were not obvious. The morphological identification results of several mouse-shaped animals were corrected. Neighbor-Joining tree showed that individuals of the same species were clustered into a single branch with high support, and two cryptic species(C.griseus and C.sokolovi) were found. DNA barcoding technology can effectively identify rat-like animals, but due to the imperfect database, traditional taxonomic identification methods and modern molecular biological identification methods still need to be combined to ensure the accuracy of identification results.
陈宝宝, 孙养信, 范锁平, 吕文, 聂守民, 李胜振, 安翠红. DNA条形码技术在陕西省鼠形动物鉴定中的应用[J]. 中国人兽共患病学报, 2020, 36(4): 325-329,339.
CHEN Bao-bao, SUN Yang-xin, FAN Suo-ping, LYU Wen, NIE Shou-ming, LI Shen-zheng, AN Cui-hong. Application for identification of Murine-like animals by DNA barcoding in Shaanxi Province. Chinese Journal of Zoonoses, 2020, 36(4): 325-329,339.
2020, Vol. 36 
