Isolation and identification of Yichang virus from Culex mosquitoes in Chongqing,China
LI Fan1, 2, FU Shi-hong1, 2, HE Xiao-zhou3, HE Ya-ming4, YE Sheng4, SHAO Nan1, 2, YIN Qi-kai1, 2, ZHAO Jie-rong1, FENG Pan-pan1, 2, LU Yang1, CHEN Xiao-jing1, HE Ying1, 2, LING Hua4, MA Xue-jun3, LIANG Guo-dong1, 2, XU Song-tao1, 2, WANG Huan-yu1, 2
1. Department of Arbovirus, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; 2. State Key Laboratory of Infectious Disease Prevention and Control, Chinese Center for Disease Control and Prevention, Beijing 102206, China; 3. Department of Core Facility, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; 4. Chongqing Center for Disease Control and Prevention, Chongqing 400042, China
Abstract:To isolate and identify viruses from mosquito samples collected in Chongqing in 2017. Tissue culture was used to isolate the virus, next generation sequencing was used generated the genome of the isolated,and phylogenetic analysis was used to analyze its characteristics. The results showed the isolated CQFD2017 can induce cytopathic effect on Aedes albopictus (C6 / 36) cell, but not Baby Hamster Kidney (BHK-21) cell and African green monkey kidney (Vero) cell. The whole genome of CQFD2017 was 20893 bp in length, containing six open reading frames (ORFs). Phylogenetic analysis showed that the strain was close related to Yichang virus of family Mesoniviridae, order Nidovirales, with 98.6% nucleic acid identity, and 99.06% and 99.15% amino acid identify of ORF1a and ORF 1b regions to Yichang Virus(NC_040534), the isolate was designated Yichang virus isolated CQFD2017. CQFD2017 strain isolated from Culex mosquitoes in Chongqing,China was identified as Yichang virus.
李樊, 付士红, 何小周, 何亚明, 叶盛, 邵楠, 殷启凯, 赵洁荣, 冯盼盼, 禄阳, 陈晓菁, 何英, 凌华, 马学军, 梁国栋, 许松涛, 王环宇. 重庆市库蚊标本中宜昌病毒的分离鉴定[J]. 中国人兽共患病学报, 2020, 36(6): 429-433.
LI Fan, FU Shi-hong, HE Xiao-zhou, HE Ya-ming, YE Sheng, SHAO Nan, YIN Qi-kai, ZHAO Jie-rong, FENG Pan-pan, LU Yang, CHEN Xiao-jing, HE Ying, LING Hua, MA Xue-jun, LIANG Guo-dong, XU Song-tao, WANG Huan-yu. Isolation and identification of Yichang virus from Culex mosquitoes in Chongqing,China. Chinese Journal of Zoonoses, 2020, 36(6): 429-433.
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