Antigen 10 of Echinococcus granulosus (Eg10) induce DC production Th2 response
WANG Ya-na1, 2, 3, WANG Chan1, WANG Shuang1
1. Department of Medical Genetic and Cell biology of academic institute in Ningxia Medical University,Yinchuan 750004, China; 2. Key labortory of hydatid disease of Ningxia Medical University, Yinchuan 750004, China; 3. Center of Scientific Technology of Ningxia Medical University, Yinchuan 750004, China
Abstract：To explore the immune mechanism of the mice immunized with Eg10 were infected with Echinococcus granulosus more severe than control group. The bone marrow dendritic cell (BMDC) of mice were obtained and cultured with different antigens including Eg10, hydatid fluid (HF) and lipopolysaccharide(LPS). The morphology of DCs in different groups were observed by electronic microscope, the capacity of up-taking, migrating, surface molecular and T cell proliferation capability were detected with flow cytometry. cytokines (IL-10,IL-6,IL-12p70 and TNF-α) in DCs supernatant were detected by Enzyme-linked immunosorbent assay (ELISA). Results showed that Eg10 like HF inhibited the DCs maturation, and the folds on superficial DCs of the two groups became more than control group and had very weak capability induce T cell proliferation, but T regulation cells increased. Eg10 group’s DCs had higher up-taking ability and expressed lower surface molecular of DCs, only MHCII and CD86 were higher than control group significantly (P<0.05); and secreted cytokines Interleukin-10 (IL-10) and IL-6 higher level than other groups. However, after added LPS DCs became semi-mature status and more dendrites grow on surface, and surface molecular MHCII、CD80、CD40 expressed higher than other groups except for LPS group, but CD86 level were decreased. Meanwhile, the level of IL-10 and IL-6 were also decreased. So, these data indicated that Eg10 inhibited DC maturation and induced DCs produce Th2 immune response.
王娅娜, 王婵, 王双. 细粒棘球绦虫Eg10诱导树突状细胞产生Th2免疫反应[J]. 中国人兽共患病学报, 2020, 36(6): 439-447.
WANG Ya-na, WANG Chan, WANG Shuang. Antigen 10 of Echinococcus granulosus (Eg10) induce DC production Th2 response. Chinese Journal of Zoonoses, 2020, 36(6): 439-447.
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