Exploration of MLVA Genotyping Application in Brucella Clinical Isolates
LYU Yan-ning1,2, CHEN Li-juan1, LI Ren-qing1, SUN Yu-lan1,CHEN Yan-wei1,WANG Quan-yi1
1. Beijing Center for Diseases Prevention and Control, Beijing Research Centre for Preventive Medicine,Beijing 100013, China; 2. School of Public Health, Capital Medical University, Beijing 100069, China
Abstract：To investigate the significance of MLVA genotyping for the discrimination of brucellosis relapse and reinfection. Brucella strains were isolated from the patients with primary and secondary onset of brucellosis. The Brucella Species identification and the MLVA-16 genotyping were carried out for the isolates, the results were then analyzed and the differences between the genotypes and each VNTR were investigated. Eight Brucella strains were isolated from four patients with double onsets of brucellosis. Seven genotypes were identified by MLVA-16 genotyping for the eight isolates. Two strains isolated from one patient with a short time interval of the double onsets was identified as the same MLVA-16 genotype, which indicated that the secondary onset was not reinfection but relapse. While the other six stains isolated from the other patients with a longer time interval of the double onsets were identified as six different MLVA-16 genotypes, and each strain was shown in a difference at one locus. The results indicated that the secondary onset of these three patients were likely reinfection but not relapse. MLVA genotyping of the isolates from the patients with double onsets of brucellosis could provide clue for the discrimination of brucellosis relapse and reinfection.
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