Cloning and sequence analysis of the partial nad1 gene within mitochondrial DNA of Echinococcus granulosus
YU Jing-feng1,GUI Zheng1,WU Lin1,YANG Xiao-ye2,WANG Rui2, MU Lan1
1. School of Basic Medical Science,Inner Mongolia Medical University,Hohhot 010110,China; 2. Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease,Ministry of Agriculture; College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China
Abstract:To further improve the molecular taxonomy of Echinococcus granulosus and provide more optimized inter and intraspecific marker genes.The mitochondrial NADH dehydrogenase 1 (nad1) gene was amplified and sequenced from the DNA of the Echinococcus granulosus extracted from infected sheep and patients in Inner Mongolia, then the phylogenetic tree was constructed using DNAStar 5.0 and identified by MEGA 4.0 softwares. As the result, the sequence length of nad1 gene of sheep and human strain amplified from E. granulosus DNA was 895 bp, which was 86.5% similar to that of E. canadensis, located in the same branch, with the highest bootstrap value of 100%. However, the branch of nad 1 sequence in the sheep and human E. granulosus was the farthest from that of Diplogonoporus balaenopterae. In conclusion, the nad1 genes were highly conserved and had interspecific differences, which can be widely used to study the inter and intraspecific genetic variation of echinococcus granulosus.
于晶峰, 桂峥, 武琳, 杨晓野, 王瑞, 木兰. 细粒棘球蚴线粒体nad1基因的克隆与序列分析[J]. 中国人兽共患病学报, 2020, 36(7): 534-538.
YU Jing-feng,GUI Zheng,WU Lin,YANG Xiao-ye,WANG Rui, MU Lan. Cloning and sequence analysis of the partial nad1 gene within mitochondrial DNA of Echinococcus granulosus. Chinese Journal of Zoonoses, 2020, 36(7): 534-538.
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