Abstract:To clarify epidemiological characteristics of diarrhea caused by Campylobacter drug-resistant condition, and molecular typing characteristics of Campylobacter in Wenzhou. Epidemiological survey was conducted in two sentinel hospitals in Wenzhou from 2017 to 2019. Stool specimens from these two hospitals were collected to isolate and culture Campylobacter. Then, conventional biochemical identification and Polymerase Chain Reaction(PCR) were used to identify the strains. At the same time, susceptibility testing and Multilocus-sequence typing(MLST) were used to investigate the strains.The detection rate of Campylobacter in 909 cases of diarrhea was 10.2%(93/909), while the positive rates of Campylobacter jejuni was 8.9%(81/909), and the positive rates of C. colon was 1.3%(12/909). There was no significant difference in Campylobacter positive rate among six age groups or different seasons (χage2=2.09,P>0.05;χsesson2=0.468,P>0.05). 22 kinds of drug resistance spectra were generated by 93 strains of Campylobacter against 11 antibacterial types, among which the majority type of spectra was Naproxinic acid (NAL)-Ciprofloxacin (CIP)-Tetracycline (TET),accounting for 38.7%(36/93). In additional, the Multiple drug resistance(MDR) rates(more than 3 kinds of antibiotics) of C. jejuni was 42.0%(34/81), and which of C. colon was 50.0% (6/12). Of all the local isolates, C. jejuni was divided into 54 sequence types(STs), containing 15 clonal complexes(CCs), and the main CC was ST45(11.1%,9/81). Meanwhile, Campylobacter colon was divided into 8 STs, and the main CC was ST828(83.3%,10/12). Totally 16 new STs were found in this study. In conclusion, Campylobacter has been the main pathogen in cases of foodborne diarrhea in Wenzhou,and showed high MDR and genetic diversity.
章乐怡, 楼辉煌, 胡玉琴, 王黎荔, 缪蔚蔚, 李毅, 吴跃进. 温州市食源性弯曲菌耐药特征与分子分型研究[J]. 中国人兽共患病学报, 2020, 36(7): 583-588.
ZHANG Le-yi, LOU Hui-huang, HU Yu-qin, WANG Li-li, MIAO Wei-wei, LI Yi, WU Yue-jin. Research on distribution, drug resistance and molecular typing of foodborne Campylobacter in Wenzhou. Chinese Journal of Zoonoses, 2020, 36(7): 583-588.
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