Genome sequencing and molecular characteristics of Listeria ivanovii subsp. ivanovii isolated from the cow feces
JIANG Hua-Ying1, 2, GAN Lin2, MAO Pan2, SONG Ze-xuan2, JI Shun-shi2, WANG Yi-qian2, WANG Yan2, XU Bao-hong3, GAO Wei-li3, CHEN Zheng-hong1, YE Chang-yun1, 2
1. Department of Microbiology, School of Basic Medical Science, Guizhou Medical University/Key Laboratory of Medical Microbiology and Parasitology of Guizhou Province, Guiyang 550004, China; 2. State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Beijing 102206, China; 3. Shijiazhuang Center for Disease Control and Prevention, Shijiazhuang 050019, China
Abstract:Listeria ivanovii, an opportunistic human pathogen, is an animal pathogen and primarily infects ruminants. Three Listeria ivanovii subsp.ivanovii strains and six Listeria innocua strains were isolated from the 196 cow feces samples with the prevalence of 1.53% and 3.06%, respectively. The main virulence genes including LIPI-1 and LIPI-2 of pathogenic Listeria were positive in three Listeria ivanovii subsp. ivanovii isolates. One incomplete prophage genes cluster and 22 drug resistance-related genes also existed in the genome of Listeria ivanovii subsp. ivanovii isolates. The results indicated that Listeria ivanovii subsp. ivanovii was carried in the cow feces. Genetic characteristics analysis of the virulence genes, resistance genes and prophage of Listeria ivanovii subsp. ivanovii isolates provide a reference for the subsp. ’s pathogenicity study.
蒋华英, 甘霖, 毛盼, 宋泽萱, 纪顺师, 王怡倩, 王艳, 徐保红, 高伟利, 陈峥宏, 叶长芸. 奶牛粪便分离的伊氏李斯特菌伊氏亚种基因组序列测定及其分子特征分析[J]. 中国人兽共患病学报, 2020, 36(8): 624-630.
JIANG Hua-Ying, GAN Lin, MAO Pan, SONG Ze-xuan, JI Shun-shi, WANG Yi-qian, WANG Yan, XU Bao-hong, GAO Wei-li, CHEN Zheng-hong, YE Chang-yun. Genome sequencing and molecular characteristics of Listeria ivanovii subsp. ivanovii isolated from the cow feces. Chinese Journal of Zoonoses, 2020, 36(8): 624-630.
[1] Esteban JI, Oporto B, Aduriz G, et al.Faecal shedding and strain diversity of Listeria monocytogenes in healthy ruminants and swine in Northern Spain[J]. BMC Vet Res, 2009, 5: 1-10. DOI: 10.1186/1746-6148-5-2 [2] Ramage CP, Low JC, Mclauchlin J, et al.Characterisation of Listeria ivanovii isolates from the UK using pulsed-field gel electrophoresis[J]. FEMS Microbiol Lett, 1999, 170(2): 349-353. DOI:10.1111/j.1574-6968.1999.tb13394.x [3] Vazquez-Villanueva J, Orgaz B, Ortiz S, et al.Predominance and persistence of a single clone of Listeria ivanovii in a Manchego cheese factory over 6 months[J]. Zoonoses Public Health, 2010, 57(6): 402-410. DOI: 10.1111/j.1863-2378.2009.01232.x [4] Jamali H, Radmehr B.Frequency, virulence genes and antimicrobial resistance of Listeria spp. isolated from bovine clinical mastitis[J]. Vet J, 2013, 198(2): 541-542. DOI: 10.1016/j.tvjl.2013. 06.012 [5] Vazquez-Boland JA, Kuhn M, Berche P, et al.Listeria pathogenesis and molecular virulence determinants[J]. Clin Microbiol Rev, 2001, 14(3): 584-640. DOI: 10.1128/CMR.14.3.584-640.2001 [6] Guillet C, Join-Lambert O, Le Monnier A, et al.Human listeriosis caused by Listeria ivanovii[J]. Emerg Infect Dis, 2010, 16(1): 136-138. DOI: 10.3201/eid1601.091155 [7] 李华信,刘宇,蒋欢.血液和骨髓同时感染伊氏李斯特菌和类志贺邻单胞菌一例[J].中华临床医师杂志, 2012, 6(2): 205-206. DOI:10.3877/cma.j.issn.1674-0785.2012.02.070 [8] Snapir Y M, Vaisbein E, Nassar F.Low virulence but potentially fatal outcome-Listeria ivanovii[J]. Eur J Intern Med, 2006, 17(4): 286-287. DOI: 10.1016/j.ejim.2005.12.006 [9] Jamali H, Paydar M, Ismail S, et al.Prevalence, antimicrobial susceptibility and virulotyping of Listeria species and Listeria monocytogenes isolated from open-air fish markets[J]. BMC Microbiol, 2015, 15: 144. DOI: 10.1186/s12866-015-0476-7 [10] Alvarez-Ordonez A, Leong D, Morgan CA, et al.Occurrence, Persistence, and Virulence Potential of Listeria ivanovii in Foods and Food Processing Environments in the Republic of Ireland[J]. Biomed Res Int, 2015, 2015: 350-526. DOI: 10.1155/2015/350526 [11] Mcauley CM, Mcmillan K, Moore SC, et al.Prevalence and characterization of foodborne pathogens from Australian dairy farm environments[J]. J Dairy Sci, 2014, 97(12): 7402-7412. DOI: 10.3168/jds.2014-8735 [12] Rocha KR, Perini HF, De Souza CM, et al.Inhibitory effect of bacteriocins from enterococci on developing and preformed biofilms of Listeria monocytogenes, Listeria ivanovii and Listeria innocua[J]. World J Microbiol Biotechnol, 2019, 35(7): 96. DOI: 10.1007/s11274-019-2675-2680 [13] Chand P, Sadana J R.Outbreak of Listeria ivanovii abortion in sheep in India[J]. Vet Rec, 1999, 145(3): 83-84. DOI: 10.1136/vr.145.3.83 [14] Alexander AV, Walker RL, Johnson BJ, et al.Bovine abortions attributable to Listeria ivanovii: four cases (1988-1990)[J]. J Am Vet Med Assoc, 1992, 200(5): 711-714. [15] Dominguez-Bernal G, Muller-Altrock S, Gonzalez-Zorn B, et al.A spontaneous genomic deletion in Listeria ivanovii identifies LIPI-2, a species-specific pathogenicity island encoding sphingomyelinase and numerous internalins[J]. Mol Microbiol, 2006, 59(2): 415-432. DOI: 10.1111/j.1365-2958. 2005.04955.x [16] Yin Y, Yao H, Doijad S, et al.A hybrid sub-lineage of Listeria monocytogenes comprising hypervirulent isolates[J]. Nat Commun, 2019, 10(1): 4283. DOI: 10.1038/s41467-019-12072-1 [17] Wang Y, Lu L, Lan R, et al.Isolation and characterization of Listeria species from rodents in natural environments in China[J]. Emerg Microbes Infect, 2017, 6(6): e44. DOI: 10.1038/emi.2017.28 [18] Cao X, Wang Y, Wang Y, et al.Prevalence and Characteristics of Listeria ivanovii Strains in Wild Rodents in China[J]. Vector Borne Zoonotic Dis, 2019, 19(1): 8-15. DOI: 10.1089/vbz.2018.2317 [19] Joensen KG, Scheutz F, Lund O, et al.Real-time whole-genome sequencing for routine typing, surveillance, and outbreak detection of verotoxigenic Escherichia coli[J]. J Clin Microbiol, 2014, 52(5): 1501-1510. DOI: 10.1128/JCM.03617-13 [20] Ge R, Mai G, Wang P, et al.CRISPRdigger: detecting CRISPRs with better direct repeat annotations[J]. Sci Rep, 2016, 6: 32942. DOI: 10.1038/srep32942 [21] Konosonoka IH, Jemeljanovs A, Osmane B, et al.Incidence of Listeria spp. in Dairy Cows Feed and Raw Milk in Latvia[J]. ISRN Vet Sci, 2012,2012:435187. DOI: 10.5402/2012/435187 [22] Dahshan H, Merwad AMA, Mohamed TS.Listeria species in broiler poultry farms: potential public health hazards[J]. J Microbiol Biotechnol, 2016, 26(9): 1551-1556. DOI: 10.4014/jmb.1603.03075 [23] Bailey GD, Vanselow BA, Hornitzky MA, et al.A study of the foodborne pathogens: Campylobacter, Listeria and Yersinia, in faeces from slaughter-age cattle and sheep in Australia[J]. Commun Dis Intell Q Rep, 2003, 27(2): 249-257. DOI:10.1353/bhm.2003.0147 [24] Linke K, Ruckerl I, Brugger K, et al.Reservoirs of listeria species in three environmental ecosystems[J]. Appl Environ Microbiol, 2014, 80(18): 5583-5592. DOI: 10.1128/AEM.01018-14 [25] Cummins AJ, Fielding AK, Mclauchlin J.Listeria ivanovii infection in a patient with AIDS[J]. J Infect, 1994, 28(1): 89-91. DOI: 10.1016/s0163-4453(94)94347-8 [26] Lessing MP, Curtis GD, Bowler IC.Listeria ivanovii infection[J]. J Infect, 1994, 29(2): 230-231. DOI: 10.1016/s0163-4453(94)90914-8 [27] Beye M, Gouriet F, Michelle C, et al.Genome analysis of Listeria ivanovii strain G770 that caused a deadly aortic prosthesis infection[J]. New Microbes New Infect, 2016, 10: 87-92. DOI: 10.1016/j.nmni.2016.01.005 [28] Elischerova K, Cupkova E, Urgeova E, et al.Isolation of Listeria ivanovii in Slovakia[J]. Cesk Epidemiol Mikrobiol Imunol, 1990, 39(4): 228-236. [29] Buchrieser C, Rusniok C, Garrido P, et al.Complete genome sequence of the animal pathogen Listeria ivanovii, which provides insights into host specificities and evolution of the genus Listeria[J]. J Bacteriol, 2011, 193(23): 6787-6788. DOI: 10.1128/JB.06120-11 [30] Gan L, Mao P, Jiang H, et al.Two Prevalent Listeria ivanovii subsp. ivanovii Clonal Strains With Different Virulence Exist in Wild Rodents and Pikas of China[J]. Front Vet Sci,2020, 7. DOI: 10.3389/fvets.2020.00088 [31] Arthur M, Molinas C, Depardieu F, et al.Characterization of Tn1546, a Tn3-related transposon conferring glycopeptide resistance by synthesis of depsipeptide peptidoglycan precursors in Enterococcus faecium BM4147[J]. J Bacteriol, 1993, 175(1): 117-127.DOI: 10.1128/jb.175.1.117- 127.1993 [32] Arthur M, Depardieu F, Molinas C, et al.The vanZ gene of Tn1546 from Enterococcus faecium BM4147 confers resistance to teicoplanin[J]. Gene, 1995, 154(1): 87-92. DOI: 10.1016/0378-1119 (94)00851-i [33] Charpentier E, Courvalin P.Antibiotic resistance in Listeria spp[J]. Antimicrob Agents Chemother, 1999, 43(9): 2103-2108. DOI:10.1016/S0399-077X(05)81060-1 [34] Williams CL, Neu HM, Gilbreath JJ, et al.Copper resistance of the emerging pathogen acinetobacter baumannii[J]. Appl Environ Microbiol, 2016, 82(20): 6174-6188. DOI: 10.1128/AEM. 01813-16 [35] Hupfeld M, Fouts DE, Loessner MJ, et al.Genome sequences of the Listeria ivanovii subsp. ivanovii type strain and two Listeria ivanovii subsp. londoniensis strains[J]. Genome Announc, 2015, 3(1):e01440-14. DOI: 10.1128/genomeA.01440-14