Abstract：To investigate the biological characteristics of human ACE2 protein, the published encoded sequence was obtained from NCBI database. Then, its structural characteristics, physicochemical properties, signal peptide, transmembrane structure and protein post-translational modification were studied. Protein interactions and its interaction networks were analyzed by STRING. The function of the interaction networks was examined, and the biological analyses were performed utilizing GO enrichment and KEGG pathway analysis via STRING database. The information of the biological processes, cellular components, molecular functions, and signaling pathways of ACE2 interacting proteins were obtained. The results showed that ACE2 gene encoding 805 amino acids, and the theoretical isoelectric point was 5.36, which indicated it was a hydrophobic unstable protein. The ACE2 protein contained a signal peptide and a transmembrane region. There were 6 potential n-glycosylation sites and 80 potential phosphorylation sites. The interacted proteins were mainly located in the cell membrane and extracellular and was involved in biological processes such as blood pressure regulation and hormone regulation. KEGG analysis revealed that the most part enriched in G protein-coupled receptor binding signal pathways, renin-angiotensin, chemokines and relaxins pathways. In this study, the possible mechanism of ACE2 protein mediating SARS-CoV-2 invasion and causing lung damage was discussed,combined with the results of bioinformatics analysis of the structure and function of human ACE2 protein. It provides a certain theoretical basis for SARS-CoV-2 virus invasion, blocking and drug development.
段瑞芳, 武翠玲, 王牛牛, 任晨霞. 人ACE2蛋白结构和功能的生物信息学分析[J]. 中国人兽共患病学报, 2020, 36(9): 712-719.
DUAN Rui-fang, WU Cui-ling, WANG Niu-niu, REN Chen-xia. Bioinformatics analysis of the structure and function of human ACE2 protein. Chinese Journal of Zoonoses, 2020, 36(9): 712-719.
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2020, Vol. 36 
