Abstract:To explore the value of simultaneous detection of multiple viruses in epidemic prevention and control of COVID-19. To analyze respiratory virus infection of 114 suspected COVID-19 patients, real-time RT-PCR was used to detect SARS-CoV-2 nucleic acid. At the same time,the thermostatic amplification was used to detect other 18 respiratory virus . As results, the nucleic acid of 114 suspected COVID-19 patients was negative, and 21 of them were infected with non-other respiratory viruses, with an infection rate of 18.42%. A total of 10 respiratory viruses were detected in 21 cases, including coronavirus NL63/229E, respiratory syncytial virus, human coxsackie virus A16, influenza B virus, human parainfluenza virus type 1, human parainfluenza virus type 3, human metapneumovirus, influenza A virus, seasonal influenza a virus subtype H3, and enterovirus/rhinovirus. There were 6 cases of influenza B virus infection and 5 cases of respiratory syncytial virus. Three patients were co-infected with two viruses: respiratory syncytial virus mixed with coxsackie virus A16, coronavirus NL63/229E mixed with human parainfluenza virus 1, and influenza A virus mixed with influenza A virus seasonal H3 subtype. In conclusion, in response to the SARS-CoV-2 epidemic, attention should be paid to the identification of SARS-CoV-2 and other respiratory viruses in suspected COVID-19 patients, so as to effectively exclude suspected cases.
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