Differential protein expression analysis on protoscolex of Echinococcus granulosus in the liver and lung of sheep
PENG Wen-jun1, CAO De-ping2, ZHANG Yao-gang3, LIU Yan4, JIANG Bo-fan4, ZHAO Hai-long4
1.The Department of Animal Surgery of Qinghai University Medical College,Xining 810001, China; 2.The Department of Human Parasitology of Bascic Medical College of Guilin Medical College,Guilin 541104, China; 3.The Echinococcosis Key Laboratory of Qinghai University, Xining 810001, China; 4.The Department of Pathogenic Biology of Qinghai University Medical College,Xining 810001, China
Abstract:To study the differential protein expression profile of the protoscolex of Echinococcus granulosus in liver and lung of sheep. In order to screening specific proteins for development and growth regulation of protoscolex. The protein was extracted from protoscolex in liver and lung of sheep from different groups and analyzed the protein profile by SDS-PAGE. And then the protein expression profiles of protoscolex between liver and lung were analyzed by iTRAQ and 2D liquid chromatography-tandem mass spectrometry (LC-MS). The differentially expressed proteins were analyzed by Mascot and GO software. In this study, a total of 1 229 meaningful protein spots were identified. Differentially expressed proteins total 217 were obtained to compare Hepatic_P with Lung_P and the trend of regulation, of which 74 were up-regulated (eg, Fatty acid binding protein, Fibrinogen alpha, Epodide hydrolase, catalase, avyl-COA sythetase, apolipoprotein A4, glycogen phosphorylase, 6-phosphogluconolactonase, acetyl-coenzyme acylthransferse and some uncharacterized proteins, and so on) and 143 were down-regulated (eg, L-lactate dehydrogenase, glutathione peroxidase, endoplasmic reticulum oxidoreductase, actin protein, myosin protein, asparaginase, ferritin, HSP90, 6-phosphoglucoate dehydrogenase, I collagen, IV collagen, vimentin, vitronectin, phospholipid transfer protein, phosphoglycerate kinase and so on). In conclusion,it was found that the protein expression of the protoscolexof E. granulosus was different in sheep liver and lung.
彭文军, 曹得萍, 张耀刚, 刘燕, 姜博璠, 赵海龙. 绵羊肝脏、肺脏细粒棘球蚴原头节差异蛋白质表达分析[J]. 中国人兽共患病学报, 2020, 36(10): 807-812.
PENG Wen-jun, CAO De-ping, ZHANG Yao-gang, LIU Yan, JIANG Bo-fan, ZHAO Hai-long. Differential protein expression analysis on protoscolex of Echinococcus granulosus in the liver and lung of sheep. Chinese Journal of Zoonoses, 2020, 36(10): 807-812.