Abstract：This study aimed to analyse changes in the intracellular protein composition of H446 small-cell lung cancer cells after their coculture with excretory-secretory proteins (ESPs) from Trichinella spiralis muscle larvae. The muscle larvae of T. spiralis were collected and used for ESPs preparation. H446 cells in the experimental group were treated with 0.85 mg/mL ESPs, while those in the control group was treated with serum-free RPMI 1640 medium. After 24 h of treatment, the total proteins were collected and extracted. Total proteins were then separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and digested by enzymatic hydrolysis. The total proteins were identified by liquid mass spectrometry (LC-MS/MS). Gene Ontology (GO) analysis was used to analyze the enrichment of cellular components, molecular functions and biological processes in proteins which expression in the experimental group differed from that in the control group. The results of LC-MS/MS showed 240 proteins with clear protein bands in the experimental group, of which 204 proteins were identified, 22 proteins were unidentified, and 14 proteins were hypothetical. Among the 204 known proteins, one anti-tumor related protein, histone 2A (histone 2A) was found. In the control group, A total of 233 proteins were found and 191 proteins were overlapped between the experimental and control groups. 49 and 42 non-overlapping proteins were found in experimental group and control group, respectively. In conclusion, the intracellular protein composition of H446 cells in the experimental group underwent complex changes after culture with ESPs, showing that the anti-tumor mechanism of ESPs and their processes are complex.
李美辰, 潘靖丹, 范泽华, 陈万楼, 陈彩铃, 吴合亮, 李天歌, 孙亚杰, 杜娈英. 旋毛虫肌幼虫ESPs作用于小细胞肺癌H446细胞蛋白组分变化分析[J]. 中国人兽共患病学报, 2020, 36(10): 821-826.
LI Mei-chen, PAN Jing-dan, FAN Ze-hua, CHEN Wan-lou, CHEN Cai-ling, WU He-liang, LI Tian-ge, SUN Ya-jie, DU Luan-ying. Changes of protein components in small cell lung cancer H446 cells treated with ESPs of Trichinella spiralis. Chinese Journal of Zoonoses, 2020, 36(10): 821-826.
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2020, Vol. 36 
