Transcriptional analysis of artificially induced rifampicin-resistant Brucella melitensis
YANG Xiao-wen, ZHAO Hong-yan, PIAO Dong-ri, TIAN Guo-zhong, JIANG Hai
State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention,Bejing 102206,China
Abstract：The aim of this study was to screen rifampicin-resistance related genes (except for the rpoB gene). This study used artificial mutagenesis technology to obtain rifampin-resistant strains. And the gene expression levels of standard and rifampin-resistant strains at the whole genome level were obtained by transcriptome sequencing to predict rifampicin metabolism-related genes and major metabolic pathways in Brucella spp. The results showed that 121 genes were up-regulated and 197 genes were down-regulated in the rifampin-resistant strain. The functions of different expressed genes were mainly concentrated in catalytic activity, membrane part, cell, metabolic process and cellular process. They were mainly involved in metabolic pathways such as carbon metabolism, biosynthesis of antibiotics, bacterial secretion system, inositol phosphate metabolism and ABC transporters. This study found that genes including the virB operon participate in the resistance to rifampicin through changing in expression levels. This study provides new insights for the screening of Brucella resistance-related genes, and also provides basic data for the prevention and control of Brucella strains.
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2020, Vol. 36 
