Abstract:To identify the characteristics and correlation of regulatory T cells (Treg), Foxp3 gene and interleukin 10 (IL-10) in brucellosis. The peripheral blood samples were collected from 20 patients with acute brucellosis, 29 patients with chronic brucellosis and 52 healthy controls from June 2019 to December 2019 in People's Hospital of Xinjiang Uygur Autonomous Region. Flow cytometry was used to detect the number of Treg cells, fluorescence quantitative PCR was used to measure the mRNA level of Foxp3 in extracted PBMC, and AimPlex method was used to determine the level of IL-10 in plasma. Finally, the correlation between the number of Treg cells, the level of Foxp3 mRNA and the level of IL-10 was analyzed. As results, compared with the normal control group, the proportion of Treg cells increased (t=2.87, P=0.01), the mRNA level of Foxp3 increased (t=3.42,P<0.01), and the IL-10 level increased (t=7.90, P<0.01) in the acute brucellosis group; similarly, and the increased number of Treg cells (t=10.82,P<0.01), the higher mRNA level of Foxp3 (t=6.89,P<0.01), and the increased IL-10 level (t=21.42, P<0.01) were also found in the chronic brucellosis group. Compared with the acute brucellosis group, the number of Treg cells increased (t=4.96, P<0.01), the mRNA level of Foxp3 increased (t=2.38, P=0.02), and the IL-10 level increased (t=7.64, P<0.01) significantly in the chronic brucellosis group. The expression level of Foxp3 mRNA and the level of IL-10 in Brucellosis group were positively correlated with the number of Treg (r=0.72, P<0.01; r=0.71, P<0.01). Thus, the phenomenon of the increased number of Treg cells, the upregulated expression of Foxp3 gene, and the increased level of IL-10 after Brucella infection, which may be the reason for the sustained infection of Brucella.
刘熔, 王昌敏, 郝立君, 卢佩佩, 李智伟. 调节性T细胞在布鲁氏菌感染中的变化特点研究[J]. 中国人兽共患病学报, 2020, 36(10): 847-851.
LIU Rong, WANG Chang-min, HAO Li-jun, LU Pei-pei, LI Zhi-wei. Study on the change characteristics of regulatory T cells in Brucella infection. Chinese Journal of Zoonoses, 2020, 36(10): 847-851.
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