Abstract：To evaluate the clinical application value of fluorescence probe PCR based on KOD polymerase technology in the diagnosis of pulmonary tuberculosis (TB). Between October 2019 and April 2020, we collected a total of 337 samples from patients with suspected TB. Each specimen was examined with smears, MGIT960 solid culture, and fluorescence probe PCR based on KOD DNA polymerase and Taq DNA polymerase. Using the clinical diagnosis as a reference standard and MGIT960 solid culture as the gold standard, we compared the results with those of fluorescence probe PCR based on Taq DNA polymerase and evaluated the performance of fluorescence probe PCR based on KOD DNA polymerase. On the basis of the diagnosis, the sensitivities of MGIT960 solid culture, and fluorescence probe PCR based on Taq DNA polymerase and KOD DNA polymerase were 71.56%, 76.15% and 76.61%, respectively. Fluorescence probe PCR based on KOD DNA polymerase produced similar results to those of MGIT960 solid culture (χ2=1.445,P=0.229,P>0.05). With the MGIT960 solid culture method result as the gold standard, the sensitivity and specificity of fluorescence probe PCR based on KOD DNA polymerase were 92.31% (144/156) and 86.39% (146/169), respectively; the sensitivity and specificity in smear negative patients were 84.21% (64/76) and 88.48% (146/165), respectively. Fluorescence probe PCR based on KOD DNA polymerase is easily performed and has relatively high sensitivity and specificity. This method can provide a basis for clinical diagnosis of pulmonary TB and is suitable for all types of laboratories.
黄明翔, 陈新朝, 陈晓红, 方美兰. KOD DNA聚合酶荧光PCR技术在肺结核诊断中的应用评价[J]. 中国人兽共患病学报, 2020, 36(10): 852-857.
HUANG Ming-xiang, CHEN Xin-chao, CHEN Xiao-hong, FANG Mei-lan. Clinical evaluation of fluorescence probe PCR based on KOD DNA polymerase technology in the diagnosis of pulmonary tuberculosis. Chinese Journal of Zoonoses, 2020, 36(10): 852-857.
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