Transcriptomic expression profiles of SPF chicken infected with duck-origin H7N9 subtype avian influenza virus
SHI Shao-hua1,2,3, CHEN Zhen1,2,3, CHENG Long-fei1,2,3, FU Guang-hua1,2,3, FU Qiu-ling1,2,3, LIU Rong-chang1,2,3, WAN Chun-he1,2,3, Chen Hong-mei1,2,3, HUANG Yu1,2,3
1. Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences,Fuzhou 350013, China; 2. Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention, Fuzhou 350013, China; 3. Animal Diseases Control Technology Development Center,Fuzhou 350013, China
Abstract:To investigate the expression changes of host genes of SPF chickens infected with duck-origin H7N9 subtype avian influenza virus. The lungs of SPF chickens infected with duck-origin H7N9 subtype avian influenza virus were collected for high-throughput sequencing. Compared with the control group, 740 genes were differentially expressed in the infection group, including 602 up-regulated genes and 138 down-regulated genes. The analysis of the GO items showed that the differentially expressed genes were mainly involved in immune responses and inflammatory responses. KEGG database comparative annotation and enrichment analysis showed that there were 7 pathways with significant enrichment, among which 11 genes were up-regulated in Toll-like pathway, namely IL-6, TLR4, Pik3, IRF7, MD-2, IRF5, MYD88, CD86, STAT1, TLR2, and CCL4. There were 7 up-regulated genes in NOD-like receptor signaling pathway, which were IRF7, CTSB, P2RX7, CYBB, PSTPIP1, HSP90AA1, and NAMPT. In Toll-like signaling pathway,TLR4 was activated by MD-2 after viral infection, and then activated downstream IRF5. At the same time, the NLRP3 inflamator also played an important role in the process of H7N9 virus infection.
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