Abstract：This article focused on studying the spectrum of multiple drug-resistant tuberculosis (MDR.tuberculosis) isolates and gene mutations in MDR.tuberculosis isolates in Xuzhou. We sought to identify molecular evidence supporting a rapid diagnostic method for MDR.tuberculosis through analyzing the relationships between the spectra of MDR.tuberculosis isolates and mutations in MDR.tuberculosis isolate genes. A total of 115 MDR.tuberculosis isolates and 66 pan-susceptible isolates were chosen via random sampling from the entire Xuzhou area. We used the gene chip technique to detect the ropB gene in RFP-resistant MDR.tuberculosis and katG, inhA and aphC of INH-resistant MDR.tuberculosis; the results were analyzed with t-tests. Nine spectra of MDR.tuberculosis treated with six drugs were identified; the most prevalent spectrum was that of INH+RFP, accounting for 47.83%, followed by INH+RFP+SM, accounting for 20.00%. Among the mutations in drug resistance genes of MDR.tuberculosis treated with INH (87.83%), 64.35% had mutations in katG, 3.48% had mutations in inhA, 12.17% had mutations in katG+inhA, and 12.17% had mutations in katG+aphc. The total mutation rate in MDR.tuberculosis isolates (115) was higher than that in pan-susceptible isolates (66) treated with INH, and the difference was statistically significant (t=107.56,P<0.05). For the other mutations, only the difference in the mutation rate in katG was statistically significant (P<0.05). The rpoB gene mutation rate of drug resistance genes in MDR.tuberculosis treated with RFP was 86.09%. The locations of mutations were at codons 531 (45.22%), 516 (8.70%), 531+516 (13.91%), 531+513 (12.17%) and 531+516+513 (3.48%), whereas 526,533 and 516+533 codon mutations were quite rare. The total mutation rate of MDR.tuberculosis isolates under RFP treatment (115) was higher than that of pan-susceptible isolates (66), and the difference was statistically significant (t=94.92, P<0.05). For the other mutations, only the difference in the mutation rate in codons 531 and 516 was statistically significant (P<0.05). Mutations under INH and RFP treatment of MDR.tuberculosis were evident and showed polymorphism and regionalism in Xuzhou. The common mutation sites in MDR.tuberculosis treated with RFP were mainly codons 531, 516 and 533; the mutation site with INH treatment was most commonly katG, which was relatively stable and may serve as a rapid and effective index for early diagnosis of MDR.tuberculosis in Xuzhou.
刘加彬, 张瑞梅, 刘成永. 徐州市耐多药结核耐药分析及分枝杆菌基因突变特征研究[J]. 中国人兽共患病学报, 2020, 36(12): 1014-1018.
LIU Jia-bin, ZHANG Rui-mei, LIU Cheng-yong. Drug-resistance analysis and characteristics of gene mutations in multiple drug-resistant tuberculosis in Xuzhou, China. Chinese Journal of Zoonoses, 2020, 36(12): 1014-1018.
2020, Vol. 36 
