Abstract：This study aimed to determine the infection rate, main species and drug resistance of nontuberculous mycobacterium (NTM) in five drug resistance monitoring locations in Henan Province, 2013-2018. A total of 1 781 mycobacterium positive cultures were initially identified by the traditional PNB/TCH growth test, then by the melting curve method and PCR sequencing. The drug susceptibility to INH, RFP, EMB, SM, KM, OFX, CPM, PTO and PAS was tested with the proportional method. Among the 1 781 samples, 67 were positive by PNB/TCH. Melting curve analysis and PCR sequencing revealed that two were M. tuberculosis. Of the 65 NTM cases, M. intracellulare accounted for 75.4% (49/65), and M. kansasii accounted for 7.7% (5/65). A small number of cases were due to M. abscess (n=3, 4.6%), M. avium (2, 3.1%) and M. septicum (1, 1.5%). M. tuberculosis and M. intracellulare co-infection accounted for 7.7% (5/65). Of the 1781 patients, the infection rate was 3.8% (53/1 388) in men and 3.1% (12/393) in women. There was no statistical difference (χ2=0.510, P=0.475). The infection rate in patients younger than 50 years old was 2.3% (20/840), and that in patients over 50 years old was 4.8% (45/941). The difference was statistically significant (χ2=7.303, P<0.01). The infection rate was 2.2% (24/1 097) in initially treated patients and 6.0% (41/684) in retreated patients; the difference was statistically significant (χ2=17.359, P<0.01). The drug resistance rate was 100% to INH and was above 80% to RFP, SM, OFX, CPM and PAS. The multi-drug resistance rate for NTM was 95.4%, and the rate of extensive drug resistance was 89.2%. Together, the results indicated that M. intracellulare was the dominant NTM strain in Henan’s drug resistance monitoring areas. The ratio of men infected with NTM was higher than that of women. Older and retreated patients were more susceptible to NTM. Finally, the NTM strains were generally resistant to common anti-tuberculosis drugs.
赵东阳, 王少华, 苏茹月, 郑丹薇, 朱岩坤, 马晓光, 石洁, 李辉, 孙定勇. 2013-2018年河南省耐药监测点非结核分枝杆菌流行特征分析[J]. 中国人兽共患病学报, 2021, 37(2): 128-132.
ZHAO Dong-yang, WANG Shao-hua, SU Ru-yue, ZHENG Dan-wei, ZHU Yan-kun, MA Xiao-guang, SHI Jie, LI Hui, SUN Ding-yong. Analysis of characteristics of nontuberculous mycobacterial infections at drug resistance monitoring points in Henan Province, 2013-2018. Chinese Journal of Zoonoses, 2021, 37(2): 128-132.
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2021, Vol. 37 
