Abstract：This study aimed to investigate the mutations in the knockdown resistance gene (kdr) in field populations of Aedes albopictus in Zhongshan, Guangdong Province, and to explore the relationship between kdr gene mutations and differences in resistance between sexes, with the goal of understanding resistance levels and providing a reference for scientific control of Ae. albopictus. Larvae and pupae of Ae. albopictus were collected from five different habitats from August to November in Zhongshan and reared to adults in the laboratory. Genomic DNA was extracted from each mosquito. The kdr gene was amplified and sequenced to analyze its mutations. Chi-square tests were used to compare the differences in kdr gene mutations between sexes in adult mosquitoes. A total of 569 Ae. albopictus were analyzed in five habitats in Zhongshan. At codon 1 532, the allele was wild-type ATC/I, and the genotype was F/F; no mutations were observed. Five alleles were observed at codon 1 534: one wild-type TTC/F allele, and four mutant TTG/L, TCC/S, TCG/S and TGC/C alleles.Eight genotypes were homozygous wild-type F/F; wild/mutant heterozygous F/L, F/S and F/C; homozygous mutant L/L and S/S; and mutant heterozygous L/S and L/C. The results showed that the differences in kdr gene mutations at codon 1534 in adult mosquitoes between sexes were not statistically significant (χ2=0.798, P>0.05). We concluded that the frequency of kdr mutations in Ae. albopictus is high in Zhongshan, thus suggesting that Ae. albopictus may be resistant to pyrethroid insecticides. No significant difference in kdr gene mutations between male and female Ae. albopictus was observed.
杨罗菊, 刘德星, 陈健, 魏晓雅, 李婷婷, 邱德义, 刘环宇. 中山市白纹伊蚊现场种群击倒抗性基因检测分析[J]. 中国人兽共患病学报, 2021, 37(2): 171-175.
YANG Luo-jü, LIU De-xing, CHEN Jian, WEI Xiao-ya, LI Ting-ting, QIU De-yi, LIU Huan-yu. Detection and analysis of the knockdown resistance gene in the field populations of Aedes albopictus in Zhongshan. Chinese Journal of Zoonoses, 2021, 37(2): 171-175.
2021, Vol. 37 
